PMID- 10556826
OWN - NLM
STAT- MEDLINE
DCOM- 19991223
LR  - 20181130
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 29
IP  - 11
DP  - 1999 Nov
TI  - Interaction of SLP adaptors with the SH2 domain of Tec family kinases.
PG  - 3702-11
AB  - Activation of lymphocytes through their antigen receptors leads to mobilization
      of intracellular Ca(2+) ions. This process requires expression of SLP adaptors
      and involves phosphorylation of phospholipase C-gamma isoforms by the Tec-related
      protein tyrosine kinase Btk in B cells and Itk in T cells. The SH2 domain of Btk 
      and Itk is essential for phospholipase C-gamma phosphorylation and mutations in
      this domain lead to the X-linked agammaglobulinemia immuno deficiency in humans. 
      Here we show that, in contrast to SH2 domains from other signaling proteins, the 
      Btk and Itk SH2 domains exhibit a restricted binding specificity. They bind
      selectively to tyrosine-phosphorylated SLP-65 and SLP-76 in activated B and T
      cells, respectively. Our findings suggest that Btk/Itk and phospholipase C-gamma 
      both bind via their SH2 domain to phosphorylated SLP adaptors, and that this
      association is required for the activation of phospholipase C-gamma.
FAU - Su, Y W
AU  - Su YW
AD  - Abteilung fur Molekulare Immunologie Biologie III, Universitat Freiburg und
      Max-Planck-Institut fur Immunbiologie, Freiburg, Germany.
FAU - Zhang, Y
AU  - Zhang Y
FAU - Schweikert, J
AU  - Schweikert J
FAU - Koretzky, G A
AU  - Koretzky GA
FAU - Reth, M
AU  - Reth M
FAU - Wienands, J
AU  - Wienands J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (B cell linker protein)
RN  - 0 (Carrier Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Receptors, Antigen, B-Cell)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (SLP-76 signal Transducing adaptor proteins)
RN  - 42HK56048U (Tyrosine)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - EC 2.7.10.1 (LTK protein, human)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (Agammaglobulinaemia Tyrosine Kinase)
RN  - EC 2.7.10.2 (BTK protein, human)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Agammaglobulinaemia Tyrosine Kinase
MH  - B-Lymphocytes/*metabolism
MH  - *Carrier Proteins
MH  - Cell Line
MH  - Glutathione Transferase/metabolism
MH  - Humans
MH  - Jurkat Cells
MH  - Phosphoproteins/isolation & purification/*metabolism
MH  - Phosphorylation
MH  - Protein-Tyrosine Kinases/isolation & purification/*metabolism
MH  - Receptor Protein-Tyrosine Kinases/*metabolism
MH  - Receptors, Antigen, B-Cell/*metabolism
MH  - Receptors, Immunologic
MH  - Recombinant Fusion Proteins/metabolism
MH  - *Signal Transduction
MH  - Tyrosine/metabolism
MH  - *src Homology Domains
EDAT- 1999/11/11 00:00
MHDA- 1999/11/11 00:01
CRDT- 1999/11/11 00:00
PHST- 1999/11/11 00:00 [pubmed]
PHST- 1999/11/11 00:01 [medline]
PHST- 1999/11/11 00:00 [entrez]
AID - 10.1002/(SICI)1521-4141(199911)29:11<3702::AID-IMMU3702>3.0.CO;2-R [doi]
PST - ppublish
SO  - Eur J Immunol. 1999 Nov;29(11):3702-11. doi:
      10.1002/(SICI)1521-4141(199911)29:11<3702::AID-IMMU3702>3.0.CO;2-R.