PMID- 10556814
OWN - NLM
STAT- MEDLINE
DCOM- 19991223
LR  - 20180423
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 29
IP  - 11
DP  - 1999 Nov
TI  - A soluble form of CTLA-4 generated by alternative splicing is expressed by
      nonstimulated human T cells.
PG  - 3596-602
AB  - CTLA-4, expressed by activated T cells, transduces an inhibitory signal. We show 
      here that PCR amplification of the coding sequence of CTLA-4 in nonstimulated
      human T lymphocytes results in the amplification of two transcripts of 650 and
      550 bp. Sequencing shows that the larger form codes for membrane CTLA-4 and the
      550-bp transcript is a spliced variant in which exon 2 coding for the
      transmembrane region is deleted. This spliced cDNA has been named CTLA-4delTM.
      The splicing induces a frame shift which results in the addition of 22 extra
      amino acids before a translational termination. Activation of T cells with
      phorbol 12-myristate 13-acetate plus ionomycin or anti-CD3 plus anti-CD28
      monoclonal antibodies induces a suppression of CTLA-4delTM mRNA expression
      associated with a preferential expression of the membrane CTLA-4 mRNA, showing
      that CTLA-4delTM mRNA expression is restricted to nonactivated T cells. A soluble
      immunoreactive form of CTLA-4 was detected in the serum of 14 / 64 healthy
      subjects. These results suggest that nonstimulated T cells may constitutively
      produce a soluble form of CTLA-4 which may have an important role in the
      regulation of immune homeostasis.
FAU - Magistrelli, G
AU  - Magistrelli G
AD  - Centre d'Immunologie Pierre Fabre, Saint Julien en Genevois, France.
FAU - Jeannin, P
AU  - Jeannin P
FAU - Herbault, N
AU  - Herbault N
FAU - Benoit De Coignac, A
AU  - Benoit De Coignac A
FAU - Gauchat, J F
AU  - Gauchat JF
FAU - Bonnefoy, J Y
AU  - Bonnefoy JY
FAU - Delneste, Y
AU  - Delneste Y
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (CTLA-4 Antigen)
RN  - 0 (CTLA4 protein, human)
RN  - 0 (Immunoconjugates)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 7D0YB67S97 (Abatacept)
SB  - IM
MH  - Abatacept
MH  - *Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, CD
MH  - Antigens, Differentiation/*biosynthesis/genetics
MH  - Base Sequence
MH  - COS Cells
MH  - CTLA-4 Antigen
MH  - Cells, Cultured
MH  - Gene Expression
MH  - Humans
MH  - *Immunoconjugates
MH  - Lymphocyte Activation
MH  - Molecular Sequence Data
MH  - RNA, Messenger
MH  - Recombinant Fusion Proteins/genetics
MH  - Solubility
MH  - T-Lymphocytes/immunology/*metabolism
EDAT- 1999/11/11 00:00
MHDA- 1999/11/11 00:01
CRDT- 1999/11/11 00:00
PHST- 1999/11/11 00:00 [pubmed]
PHST- 1999/11/11 00:01 [medline]
PHST- 1999/11/11 00:00 [entrez]
AID - 10.1002/(SICI)1521-4141(199911)29:11<3596::AID-IMMU3596>3.0.CO;2-Y [doi]
PST - ppublish
SO  - Eur J Immunol. 1999 Nov;29(11):3596-602. doi:
      10.1002/(SICI)1521-4141(199911)29:11<3596::AID-IMMU3596>3.0.CO;2-Y.