PMID- 10556625
OWN - NLM
STAT- MEDLINE
DCOM- 19991209
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1413
IP  - 3
DP  - 1999 Nov 10
TI  - Targeted disruption of the gene encoding the proteolipid subunit of mouse
      vacuolar H(+)-ATPase leads to early embryonic lethality.
PG  - 130-8
AB  - Vacuolar H(+)-ATPase (V-ATPase) is responsible for acidification of intracellular
      compartments in eukaryotic cells. Its 16-kDa subunit (proteolipid, PL16) plays a 
      central role in V-ATPase function, forming the principal channel via which
      protons are translocated. To elucidate physiological roles of V-ATPase in
      mammalian cell function and embryogenesis, we attempted to generate a PL16 null
      mutant mouse by gene-targeting. Mice heterozygous (PL16(+/-)) for the proteolipid
      mutation were intercrossed and their offspring were classified according to
      genotype. There were no homozygous (PL16(-/-)) pups among 69 neonates examined,
      but a few PL16(-/-) embryos were found during the pre-implantation stages of
      embryonic development, up to day 3.5 post-coitum. These results suggested that
      PL16 (and hence V-ATPase) may play an essential role in cell proliferation and
      viability during early embryogenesis. PL16(+/-) mice were indistinguishable from 
      their wild-type littermates and displayed no discernible abnormalities, although 
      the PL16 mRNA level in PL16(+/-) mice decreased to about one-half of wild-type
      levels.
FAU - Inoue, H
AU  - Inoue H
AD  - Department of Biological Science, Graduate School of Science, Osaka University,
      Machikaneyama-cho 1-16, Toyonaka city, Osaka, Japan.
FAU - Noumi, T
AU  - Noumi T
FAU - Nagata, M
AU  - Nagata M
FAU - Murakami, H
AU  - Murakami H
FAU - Kanazawa, H
AU  - Kanazawa H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Proteolipids)
RN  - 0 (RNA, Messenger)
RN  - EC 3.6.1.- (Vacuolar Proton-Translocating ATPases)
RN  - EC 3.6.3.14 (Proton-Translocating ATPases)
SB  - IM
MH  - Animals
MH  - Cell Division
MH  - Cell Line
MH  - Cell Survival
MH  - Crosses, Genetic
MH  - Embryo Loss/*genetics
MH  - Genotype
MH  - Heterozygote
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mutation
MH  - Proteolipids/*genetics
MH  - Proton-Translocating ATPases/chemistry/*genetics
MH  - RNA, Messenger/metabolism
MH  - *Vacuolar Proton-Translocating ATPases
EDAT- 1999/11/11 00:00
MHDA- 1999/11/11 00:01
CRDT- 1999/11/11 00:00
PHST- 1999/11/11 00:00 [pubmed]
PHST- 1999/11/11 00:01 [medline]
PHST- 1999/11/11 00:00 [entrez]
AID - S0005-2728(99)00096-1 [pii]
AID - 10.1016/s0005-2728(99)00096-1 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Nov 10;1413(3):130-8. doi:
      10.1016/s0005-2728(99)00096-1.