PMID- 10556521
OWN - NLM
STAT- MEDLINE
DCOM- 19991222
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 460
IP  - 3
DP  - 1999 Nov 5
TI  - Cloning and functional characterization of the human sodium-dependent vitamin C
      transporters hSVCT1 and hSVCT2.
PG  - 480-4
AB  - Two sodium-dependent vitamin C transporters, hSVCT1 and hSVCT2, were cloned from 
      a human kidney cDNA library. hSVCT1 had a 1797 bp open reading frame encoding a
      598 amino acid polypeptide. The 1953 bp open reading frame of hSVCT2 encoded a
      650 amino acid polypeptide. Using a Xenopus laevis oocyte expression system, both
      transporters were functionally expressed. By Eadie-Hofstee transformation the
      apparent K(m) of hSVCT1 for ascorbate was 252.0 microM and of hSVCT2 for
      ascorbate was 21.3 microM. Both transporters were sodium-dependent and did not
      transport dehydroascorbic acid. Incubation of oocytes expressing either
      transporter with phorbol 12-myristate 13-acetate (PMA) inhibited ascorbate
      transport activity. Availability of the human transporter clones may facilitate
      new strategies for determining vitamin C intake.
FAU - Daruwala, R
AU  - Daruwala R
AD  - Molecular and Clinical Nutrition Section, Bldg. 10, Rm. 4D52, MSC 1372, National 
      Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of
      Health, Bethesda, MD 20892-1372, USA.
FAU - Song, J
AU  - Song J
FAU - Koh, W S
AU  - Koh WS
FAU - Rumsey, S C
AU  - Rumsey SC
FAU - Levine, M
AU  - Levine M
LA  - eng
SI  - GENBANK/AJ269477
SI  - GENBANK/AJ269478
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Carrier Proteins)
RN  - 0 (Organic Anion Transporters, Sodium-Dependent)
RN  - 0 (Proteins)
RN  - 0 (Sodium-Coupled Vitamin C Transporters)
RN  - 0 (Symporters)
RN  - 63X7MBT2LQ (Bucladesine)
RN  - 9NEZ333N27 (Sodium)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Ascorbic Acid/*metabolism
MH  - Base Sequence
MH  - Biological Transport
MH  - Bucladesine/pharmacology
MH  - Carrier Proteins/biosynthesis/*chemistry/*genetics/physiology
MH  - Cloning, Molecular
MH  - Female
MH  - Humans
MH  - Molecular Sequence Data
MH  - Oocytes/metabolism
MH  - *Organic Anion Transporters, Sodium-Dependent
MH  - Protein Biosynthesis
MH  - Proteins/*chemistry/*genetics/physiology
MH  - Sodium/*physiology
MH  - Sodium-Coupled Vitamin C Transporters
MH  - *Symporters
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Xenopus laevis
EDAT- 1999/11/11 00:00
MHDA- 1999/11/11 00:01
CRDT- 1999/11/11 00:00
PHST- 1999/11/11 00:00 [pubmed]
PHST- 1999/11/11 00:01 [medline]
PHST- 1999/11/11 00:00 [entrez]
AID - S0014-5793(99)01393-9 [pii]
AID - 10.1016/s0014-5793(99)01393-9 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Nov 5;460(3):480-4. doi: 10.1016/s0014-5793(99)01393-9.