PMID- 10556483
OWN - NLM
STAT- MEDLINE
DCOM- 19991227
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1461
IP  - 1
DP  - 1999 Nov 9
TI  - Human Na(+)-dependent vitamin C transporter 1 (hSVCT1): primary structure,
      functional characteristics and evidence for a non-functional splice variant.
PG  - 1-9
AB  - We report here on the cloning and functional characterization of human
      Na(+)-dependent vitamin C transporter 1 (SVCT1). The human SVCT1 cDNA, obtained
      from a Caco2 cell cDNA library, encodes a protein of 598 amino acids with 12
      putative transmembrane domains. The SVCT1-specific transcript, 2.4 kb in size, is
      expressed in kidney, liver, small intestine, thymus and prostate. When expressed 
      heterologously in HRPE cells, SVCT1 mediates the transport of ascorbate, the
      reduced form of vitamin C, in a Na(+)-dependent manner. The transporter is
      specific for ascorbate with a K(t) of approximately 75 microM. The relationship
      between the cDNA-specific uptake rate of ascorbate and Na(+) concentration is
      sigmoidal with a Na(+):ascorbate stoichiometry of 2:1, indicating that the
      transport process is electrogenic. In Caco2 cells and in normal human intestine, 
      SVCT1 also exists as a non-functional splice variant with a four amino acid
      sequence inserted between E-155 and V-156. The splice variant results from the
      use of a donor site 12 bp downstream of the normal donor site.
FAU - Wang, H
AU  - Wang H
AD  - Department of Biochemistry, Medical College of Georgia, Augusta, GA, USA.
FAU - Dutta, B
AU  - Dutta B
FAU - Huang, W
AU  - Huang W
FAU - Devoe, L D
AU  - Devoe LD
FAU - Leibach, F H
AU  - Leibach FH
FAU - Ganapathy, V
AU  - Ganapathy V
FAU - Prasad, P D
AU  - Prasad PD
LA  - eng
SI  - GENBANK/AF170911
GR  - HD 33347/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (DNA, Complementary)
RN  - 0 (Organic Anion Transporters, Sodium-Dependent)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (SLC23A1 protein, human)
RN  - 0 (Sodium-Coupled Vitamin C Transporters)
RN  - 0 (Symporters)
RN  - 451W47IQ8X (Sodium Chloride)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Amino Acid Sequence
MH  - Ascorbic Acid/metabolism
MH  - Base Sequence
MH  - Biological Transport/drug effects
MH  - Caco-2 Cells
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA, Complementary/biosynthesis/chemistry
MH  - Gene Library
MH  - Humans
MH  - Intestinal Mucosa/metabolism
MH  - Kinetics
MH  - Molecular Sequence Data
MH  - *Organic Anion Transporters, Sodium-Dependent
MH  - Proteins/analysis/*chemistry/genetics
MH  - RNA Splicing
MH  - RNA, Messenger/isolation & purification
MH  - Sodium Chloride/*pharmacology
MH  - Sodium-Coupled Vitamin C Transporters
MH  - Substrate Specificity
MH  - *Symporters
MH  - Transfection
EDAT- 1999/11/11 00:00
MHDA- 1999/11/11 00:01
CRDT- 1999/11/11 00:00
PHST- 1999/11/11 00:00 [pubmed]
PHST- 1999/11/11 00:01 [medline]
PHST- 1999/11/11 00:00 [entrez]
AID - S0005-2736(99)00182-0 [pii]
AID - 10.1016/s0005-2736(99)00182-0 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Nov 9;1461(1):1-9. doi: 10.1016/s0005-2736(99)00182-0.