PMID- 10556423
OWN - NLM
STAT- MEDLINE
DCOM- 20000127
LR  - 20190905
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 10
IP  - 11
DP  - 1999 Nov
TI  - Identification and characterization of the mouse MutS homolog 5: Msh5.
PG  - 1054-61
AB  - We have identified and characterized the complete cDNA and gene for the mouse
      MutS homolog 5 (Msh5), as a step toward understanding the molecular genetic
      mechanisms involved in the biological function of this new MutS homologous
      protein in mammals. The Msh5 cDNA contains a 2502-bp open reading frame (ORF)
      that encodes an 833-amino acid protein with a predicted molecular weight of 92.6 
      kDa, which shares 89.8% amino acid sequence identity with the human hMSH5
      protein. Northern blot analysis demonstrated the presence of a Msh5 mRNA
      approximately 2.9-kb in length, most abundantly expressed in mouse testis. Yeast 
      two-hybrid analysis indicated that the mouse Msh5 protein positively interacted
      with the human hMSH4 protein-suggesting that Msh5 shares common functional
      properties with its human counterpart. Sequence and structural analyses show that
      the mouse gene Msh5 spans approximately 18 kb and contains 24 exons that range in
      length from 36 bp for exon 7 to 392 bp for exon 1. Structural comparison with the
      human hMSH5 gene revealed that all of the Msh5 internal exons, but not introns,
      are conserved in length with the human hMSH5. The Msh5 gene is located on mouse
      Chromosome (Chr) 17 in a location that is syntenic to the region of human Chr 6
      harboring the hMSH5 gene. The identification and characterization of Msh5 will
      facilitate studies of the potential functional roles of this new member of the
      MutS family.
FAU - Her, C
AU  - Her C
AD  - Life Sciences Division and Center for Human Genome Studies, Mail Stop: M888, Los 
      Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
FAU - Wu, X
AU  - Wu X
FAU - Wan, W
AU  - Wan W
FAU - Doggett, N A
AU  - Doggett NA
LA  - eng
SI  - GENBANK/AF146227
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
RN  - 0 (Bacterial Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (MSH5 protein, human)
RN  - 0 (Msh5 protein, mouse)
RN  - 0 (Proteins)
RN  - 63231-63-0 (RNA)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.1.3 (MutS DNA Mismatch-Binding Protein)
RN  - EC 3.6.1.3 (MutS protein, E coli)
SB  - IM
MH  - Adenosine Triphosphatases/genetics
MH  - Amino Acid Sequence/genetics
MH  - Animals
MH  - Bacterial Proteins/genetics
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cell Cycle Proteins
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - DNA, Complementary
MH  - *DNA-Binding Proteins
MH  - Dimerization
MH  - *Escherichia coli Proteins
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - MutS DNA Mismatch-Binding Protein
MH  - Protein Biosynthesis
MH  - Proteins/*genetics/metabolism
MH  - RNA/analysis
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Testis/metabolism
EDAT- 1999/11/11 00:00
MHDA- 1999/11/11 00:01
CRDT- 1999/11/11 00:00
PHST- 1999/11/11 00:00 [pubmed]
PHST- 1999/11/11 00:01 [medline]
PHST- 1999/11/11 00:00 [entrez]
AID - MG99-685 [pii]
AID - 10.1007/s003359901161 [doi]
PST - ppublish
SO  - Mamm Genome. 1999 Nov;10(11):1054-61. doi: 10.1007/s003359901161.