PMID- 10556305
OWN - NLM
STAT- MEDLINE
DCOM- 20000124
LR  - 20190513
IS  - 0964-6906 (Print)
IS  - 0964-6906 (Linking)
VI  - 8
IP  - 13
DP  - 1999 Dec
TI  - A novel RNA-binding nuclear protein that interacts with the fragile X mental
      retardation (FMR1) protein.
PG  - 2557-66
AB  - Silenced expression of the FMR1 gene is responsible for the fragile X syndrome.
      The FMR1 gene codes for an RNA binding protein (FMRP), which can shuttle between 
      the nucleus and the cytoplasm and is found associated to polysomes in the
      cytoplasm. By two-hybrid assay in yeast, we identified a novel protein
      interacting with FMRP: nuclear FMRP interacting protein (NUFIP). NUFIP mRNA
      expression is strikingly similar to that of the FMR1 gene in neurones of cortex, 
      hippocampus and cerebellum. At the subcellular level, NUFIP colocalizes with
      nuclear isoforms of FMRP in a dot-like pattern. NUFIP presents a C2H2 zinc finger
      motif and a nuclear localization signal, but has no homology to known proteins
      and shows RNA binding activity in vitro. NUFIP does not interact with the FMRP
      homologues encoded by the FXR1 and FXR2 genes. Thus, these results indicate a
      specific nuclear role for FMRP.
FAU - Bardoni, B
AU  - Bardoni B
AD  - Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS/INSERM/ULP, 
      BP 163, 67404 Illkirch Cedex, CU de Strasbourg, France.
      bardoni@igbmc.u-strasbg.fr
FAU - Schenck, A
AU  - Schenck A
FAU - Mandel, J L
AU  - Mandel JL
LA  - eng
SI  - GENBANK/AF159548
SI  - GENBANK/AF159549
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hum Mol Genet
JT  - Human molecular genetics
JID - 9208958
RN  - 0 (FMR1 protein, human)
RN  - 0 (Fmr1 protein, mouse)
RN  - 0 (NUFIP1 protein, human)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 139135-51-6 (Fragile X Mental Retardation Protein)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brain/metabolism
MH  - COS Cells
MH  - Cell Nucleus/metabolism
MH  - Fluorescent Antibody Technique
MH  - Fragile X Mental Retardation Protein
MH  - Fragile X Syndrome/*genetics
MH  - Gene Expression
MH  - HeLa Cells
MH  - Humans
MH  - In Situ Hybridization
MH  - Intellectual Disability/*genetics
MH  - Mice
MH  - Microscopy, Confocal
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - Nuclear Proteins/genetics/*metabolism
MH  - RNA-Binding Proteins/genetics/*metabolism
MH  - Recombinant Proteins/metabolism
MH  - Two-Hybrid System Techniques
MH  - Yeasts/genetics
MH  - Zinc Fingers
EDAT- 1999/11/11 00:00
MHDA- 1999/11/11 00:01
CRDT- 1999/11/11 00:00
PHST- 1999/11/11 00:00 [pubmed]
PHST- 1999/11/11 00:01 [medline]
PHST- 1999/11/11 00:00 [entrez]
AID - ddc290 [pii]
AID - 10.1093/hmg/8.13.2557 [doi]
PST - ppublish
SO  - Hum Mol Genet. 1999 Dec;8(13):2557-66. doi: 10.1093/hmg/8.13.2557.