PMID- 10555141
OWN - NLM
STAT- MEDLINE
DCOM- 19991123
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 99
IP  - 3
DP  - 1999 Oct 29
TI  - DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation
      and mammalian development.
PG  - 247-57
AB  - The establishment of DNA methylation patterns requires de novo methylation that
      occurs predominantly during early development and gametogenesis in mice. Here we 
      demonstrate that two recently identified DNA methyltransferases, Dnmt3a and
      Dnmt3b, are essential for de novo methylation and for mouse development.
      Inactivation of both genes by gene targeting blocks de novo methylation in ES
      cells and early embryos, but it has no effect on maintenance of imprinted
      methylation patterns. Dnmt3a and Dnmt3b also exhibit nonoverlapping functions in 
      development, with Dnmt3b specifically required for methylation of centromeric
      minor satellite repeats. Mutations of human DNMT3B are found in ICF syndrome, a
      developmental defect characterized by hypomethylation of pericentromeric repeats.
      Our results indicate that both Dnmt3a and Dnmt3b function as de novo
      methyltransferases that play important roles in normal development and disease.
FAU - Okano, M
AU  - Okano M
AD  - Cardiovascular Research Center, Massachusetts General Hospital, Department of
      Medicine, Harvard Medical School, Charlestown 02129, USA.
FAU - Bell, D W
AU  - Bell DW
FAU - Haber, D A
AU  - Haber DA
FAU - Li, E
AU  - Li E
LA  - eng
GR  - CA82389/CA/NCI NIH HHS/United States
GR  - GM52106/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases)
RN  - EC 2.1.1.37 (DNA methyltransferase 3A)
RN  - EC 2.1.1.37 (DNA methyltransferase 3B)
SB  - IM
MH  - Animals
MH  - Blastocyst/*physiology
MH  - Centromere/genetics
MH  - Crosses, Genetic
MH  - DNA (Cytosine-5-)-Methyltransferases/deficiency/*genetics/*metabolism
MH  - DNA Methylation
MH  - Homozygote
MH  - Humans
MH  - Immunologic Deficiency Syndromes/genetics
MH  - Male
MH  - Mammals
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Repetitive Sequences, Nucleic Acid
MH  - Stem Cells/physiology
EDAT- 1999/11/11 00:00
MHDA- 1999/11/11 00:01
CRDT- 1999/11/11 00:00
PHST- 1999/11/11 00:00 [pubmed]
PHST- 1999/11/11 00:01 [medline]
PHST- 1999/11/11 00:00 [entrez]
AID - S0092-8674(00)81656-6 [pii]
AID - 10.1016/s0092-8674(00)81656-6 [doi]
PST - ppublish
SO  - Cell. 1999 Oct 29;99(3):247-57. doi: 10.1016/s0092-8674(00)81656-6.