PMID- 1055415
OWN - NLM
STAT- MEDLINE
DCOM- 19750804
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 72
IP  - 4
DP  - 1975 Apr
TI  - Role of epithelial architecture and intracellular metabolism in proline uptake
      and transtubular reclamation in PRO/re mouse kidney.
PG  - 1431-5
AB  - The homozygous PRO/Re mouse has less than 1 percent of the very high proline
      oxidase activity that characterizes normal kidney cortex. In PRO/Re mouse the
      endogenous proline concentration is eight times normal in plasma and four times
      normal in kidney cortex cell, but 50 times normal in urine. The integrity of the 
      membrane transport systems for proline uptake at the antiluminal surface of
      absorbing epithelium is retained in PRO/Re kidney, as determined by the slice
      method. Clearance studies in vivo under steady-state conditions indicate that the
      integrity of the luminal uptake system shared by glycine and proline, and serving
      proline absorption, is also intact. The exaggerated renal clearance of proline in
      PRO/Re mice (50 times normal) is explained when its raised intracellular
      concentration, caused by impaired proline oxidation, is considered. Backflux into
      urine flowing down the nephron will occur under these conditions, thus impairing 
      net reclamation of proline in PRO/Re kidney. The findings reveal that membrane
      transport and intracellular metabolism of a substrate are, indeed, independent
      functions, but that metabolism of a substance can influence its transcellular
      transport.
FAU - Scriver, C R
AU  - Scriver CR
FAU - McInnes, R R
AU  - McInnes RR
FAU - Mohyuddin, F
AU  - Mohyuddin F
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 9DLQ4CIU6V (Proline)
SB  - IM
MH  - Animals
MH  - Biological Transport
MH  - Epithelial Cells
MH  - Epithelium/metabolism/ultrastructure
MH  - Glomerular Filtration Rate
MH  - Humans
MH  - Kidney Cortex/*metabolism
MH  - Kidney Tubules/*metabolism
MH  - Kinetics
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Proline/*metabolism
MH  - Rats
MH  - Species Specificity
PMC - PMC432549
EDAT- 1975/04/01 00:00
MHDA- 1975/04/01 00:01
CRDT- 1975/04/01 00:00
PHST- 1975/04/01 00:00 [pubmed]
PHST- 1975/04/01 00:01 [medline]
PHST- 1975/04/01 00:00 [entrez]
AID - 10.1073/pnas.72.4.1431 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1975 Apr;72(4):1431-5. doi: 10.1073/pnas.72.4.1431.