PMID- 10554006
OWN - NLM
STAT- MEDLINE
DCOM- 19991202
LR  - 20061115
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 59
IP  - 21
DP  - 1999 Nov 1
TI  - The nuclear pore complex protein Nup88 is overexpressed in tumor cells.
PG  - 5408-11
AB  - It has been previously shown (J. Schneider et al., Br. J. Cancer, 77: 1015-1020, 
      1998) that an antibody directed against Candida albicans proteins (C6) recognizes
      specifically a protein in human ovarian tumors. We have now performed an
      immunoscreening of a human cDNA library using this antibody and identified the
      antigen as Nup88, a protein localized preferentially at the nuclear membrane and 
      probably implicated in nucleocytoplasmic transport. Our results show that Nup88
      is strongly expressed in a series of human tumor cell lines compared with
      nontransformed cell lines at the RNA and the protein levels. Furthermore, we
      observed that in 76% of 21 ovarian tumors analyzed, the protein is also
      overexpressed in malignant tissue when compared with healthy adjacent tissue.
      Nup88 may, therefore, be considered as a putative marker for tumor growth and is 
      probably related to increased cell cycling.
FAU - Martinez, N
AU  - Martinez N
AD  - Deutsches Krebsforschungszentrum, 69120-Heidelberg, Germany.
FAU - Alonso, A
AU  - Alonso A
FAU - Moragues, M D
AU  - Moragues MD
FAU - Ponton, J
AU  - Ponton J
FAU - Schneider, J
AU  - Schneider J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Proteins)
RN  - 0 (NUP88 protein, human)
RN  - 0 (Nuclear Pore Complex Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - DNA, Complementary/analysis
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Gene Library
MH  - HL-60 Cells
MH  - HeLa Cells
MH  - Humans
MH  - Immunohistochemistry
MH  - Jurkat Cells
MH  - Membrane Proteins/genetics/*metabolism
MH  - Neoplasms/*genetics
MH  - Neoplasms, Glandular and Epithelial/metabolism
MH  - *Nuclear Pore Complex Proteins
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Ovarian Neoplasms/metabolism
MH  - RNA, Messenger/metabolism
MH  - Tumor Cells, Cultured
EDAT- 1999/12/20 00:00
MHDA- 1999/12/20 00:01
CRDT- 1999/12/20 00:00
PHST- 1999/12/20 00:00 [pubmed]
PHST- 1999/12/20 00:01 [medline]
PHST- 1999/12/20 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1999 Nov 1;59(21):5408-11.