PMID- 10553117
OWN - NLM
STAT- MEDLINE
DCOM- 19991215
LR  - 20131121
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 415
IP  - 3
DP  - 1999 Dec 20
TI  - Urocortin expression in rat brain: evidence against a pervasive relationship of
      urocortin-containing projections with targets bearing type 2 CRF receptors.
PG  - 285-312
AB  - Histochemical and axonal transport methods were used to clarify the central
      organization of cells and fibers that express urocortin (UCN), a recently
      discovered corticotropin-releasing factor (CRF)-related neuropeptide, which has
      been proposed as an endogenous ligand for type 2 CRF receptors (CRF-R2). Neurons 
      that display both UCN mRNA and peptide expression were found to be centered in
      the Edinger-Westphal (EW), lateral superior olivary (LSO), and supraoptic nuclei;
      lower levels of expression are seen in certain cranial nerve and spinal
      motoneurons and in small populations of neurons in the forebrain. Additional
      sites of UCN mRNA and peptide expression detected only in colchicine-treated rats
      are considered to be minor ones. UCN-immunoreactive projections in brain are
      predominantly descending and largely consistent with central projections
      attributed to the EW and LSO, targeting principally accessory optic,
      precerebellar, and auditory structures, as well as the spinal intermediate gray. 
      Although neither the EW nor LSO are known to project to the forebrain, UCN-ir
      neurons in the EW were identified that project to the lateral septal nucleus,
      which houses a prominent UCN-ir terminal field. Although substantial UCN-ir
      projections were observed to several brainstem cell groups that express CRF-R2,
      including the dorsal raphe and interpeduncular nuclei and the nucleus of the
      solitary tract (NTS), most prominent seats of CRF-R2 expression were found to
      contain inputs immunopositive for piscine urotensin I, but not rat UCN. The
      results define a central UCN system whose organization suggests a principal
      involvement in motor control and sensorimotor integration; its participation in
      stress-related mechanisms would appear to derive principally by virtue of
      projections to the spinal intermediolateral column, the NTS, and the
      paraventricular nucleus. Several observations, including the lack of a pervasive 
      relationship of UCN-ir projections with CRF-R2-expressing targets, support the
      existence of still additional CRF-related peptides in mammalian brain.
CI  - Copyright 1999 Wiley-Liss, Inc.
FAU - Bittencourt, J C
AU  - Bittencourt JC
AD  - Laboratory of Neuronal Structure and Function, The Salk Institute for Biological 
      Studies and Foundation for Medical Research, La Jolla, California 92037, USA.
FAU - Vaughan, J
AU  - Vaughan J
FAU - Arias, C
AU  - Arias C
FAU - Rissman, R A
AU  - Rissman RA
FAU - Vale, W W
AU  - Vale WW
FAU - Sawchenko, P E
AU  - Sawchenko PE
LA  - eng
GR  - DK-26741/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (CRF receptor type 2)
RN  - 0 (Immune Sera)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Corticotropin-Releasing Hormone)
RN  - 0 (Urocortins)
RN  - 9015-71-8 (Corticotropin-Releasing Hormone)
RN  - SML2Y3J35T (Colchicine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding, Competitive
MH  - Brain/anatomy & histology/cytology/drug effects/*metabolism
MH  - Colchicine/pharmacology
MH  - Corticotropin-Releasing Hormone/genetics/immunology/*metabolism/physiology
MH  - Female
MH  - *Gene Expression/drug effects
MH  - Immune Sera
MH  - Male
MH  - Molecular Sequence Data
MH  - Neural Pathways/metabolism/physiology
MH  - Neurons/drug effects/*metabolism/physiology
MH  - RNA, Messenger/analysis/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Corticotropin-Releasing Hormone/chemistry/*metabolism
MH  - Spinal Cord/cytology/metabolism
MH  - Stress, Physiological/physiopathology
MH  - Urocortins
EDAT- 1999/12/20 00:00
MHDA- 1999/12/20 00:01
CRDT- 1999/12/20 00:00
PHST- 1999/12/20 00:00 [pubmed]
PHST- 1999/12/20 00:01 [medline]
PHST- 1999/12/20 00:00 [entrez]
AID - 10.1002/(SICI)1096-9861(19991220)415:3<285::AID-CNE1>3.0.CO;2-0 [pii]
PST - ppublish
SO  - J Comp Neurol. 1999 Dec 20;415(3):285-312.