PMID- 10553000
OWN - NLM
STAT- MEDLINE
DCOM- 20000119
LR  - 20181130
IS  - 0022-2275 (Print)
IS  - 0022-2275 (Linking)
VI  - 40
IP  - 11
DP  - 1999 Nov
TI  - Apolipoprotein B-related gene expression and ultrastructural characteristics of
      lipoprotein secretion in mouse yolk sac during embryonic development.
PG  - 1967-77
AB  - In mice, the yolk sac appears to play a crucial role in nourishing the developing
      embryo, especially during embryonic days (E) 7;-10. Lipoprotein synthesis and
      secretion may be essential for this function: embryos lacking apolipoprotein
      (apo) B or microsomal triglyceride transfer protein (MTP), both of which
      participate in the assembly of triglyceride-rich lipoproteins, are apparently
      defective in their ability to export lipoproteins from yolk sac endoderm cells
      and die during mid-gestation. We therefore analyzed the embryonic expression of
      apoB, MTP, and alpha-tocopherol transfer protein (alpha-TTP), which have been
      associated with the assembly and secretion of apoB-containing lipoproteins in the
      adult liver, at different developmental time points. MTP expression or activity
      was found in the yolk sac and fetal liver, and low levels of activity were
      detected in E18.5 placentas. alpha-TTP mRNA and protein were detectable in the
      fetal liver, but not in the yolk sac or placenta. Ultrastructural analysis of
      yolk sac visceral endoderm cells demonstrated nascent VLDL within the luminal
      spaces of the rough endoplasmic reticulum and Golgi apparatus at E7.5 and E8.5.
      The particles were reduced in diameter at E13.5 and reduced in number at
      E18.5;-19. The data support the hypothesis that the yolk sac plays a vital role
      in providing lipids and lipid-soluble nutrients to embryos during the early
      phases (E7;-10) of mouse development. secretion in mouse yolk sac during
      embryonic development.
FAU - Terasawa, Y
AU  - Terasawa Y
AD  - Gladstone Institute of Cardiovascular Disease, University of California, San
      Francisco, CA 94110, USA.
FAU - Cases, S J
AU  - Cases SJ
FAU - Wong, J S
AU  - Wong JS
FAU - Jamil, H
AU  - Jamil H
FAU - Jothi, S
AU  - Jothi S
FAU - Traber, M G
AU  - Traber MG
FAU - Packer, L
AU  - Packer L
FAU - Gordon, D A
AU  - Gordon DA
FAU - Hamilton, R L
AU  - Hamilton RL
FAU - Farese, R V Jr
AU  - Farese RV Jr
LA  - eng
GR  - HL60844/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Lipid Res
JT  - Journal of lipid research
JID - 0376606
RN  - 0 (Apolipoproteins B)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (alpha-tocopherol transfer protein)
RN  - 0 (microsomal triglyceride transfer protein)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - Apolipoproteins B/*genetics/*metabolism
MH  - Carrier Proteins/genetics/metabolism
MH  - DNA, Complementary
MH  - Embryonic and Fetal Development/physiology
MH  - Female
MH  - Gene Expression
MH  - Immunoblotting
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Electron
MH  - RNA/analysis/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Time Factors
MH  - Tissue Distribution/genetics
MH  - Yolk Sac/*embryology/*metabolism/ultrastructure
EDAT- 1999/12/20 09:00
MHDA- 2001/03/28 10:01
CRDT- 1999/12/20 09:00
PHST- 1999/12/20 09:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1999/12/20 09:00 [entrez]
PST - ppublish
SO  - J Lipid Res. 1999 Nov;40(11):1967-77.