PMID- 10552302
OWN - NLM
STAT- MEDLINE
DCOM- 20000113
LR  - 20191103
IS  - 0949-944X (Print)
IS  - 0949-944X (Linking)
VI  - 209
IP  - 10
DP  - 1999 Oct
TI  - Molecular and genetic analysis of the Tribolium Ultrabithorax ortholog,
      Ultrathorax.
PG  - 608-19
AB  - Ultrabithorax (Ubx) is essential for the proper patterning of the posterior
      thorax and anterior abdomen in Drosophila. The Coleoptera and Diptera differ in
      the organization and structure of their thorax and anterior abdomen. Changes in
      the regulation of Ubx and/or its downstream target genes are predicted to
      underlie these altered morphologies. We exploited the feasibility of genetic
      analysis in the red flour beetle, Tribolium castaneum, to examine the role of its
      Ubx ortholog in development. We analyzed genomic and cDNA clones that predict a
      polypeptide with nearly 100% identity with the Drosophila Ubx gene in the
      homeodomain and flanking sequences. Southern blot analysis indicates that these
      clones represent DNA sequences within the Homeotic complex (HOM-C) of Tribolium. 
      Phenotypic analysis of mutant variants of the Ultrathorax (Utx) gene, and its
      location within the beetle HOM-C, strongly supports Utx being the Tribolium
      ortholog of Ubx. The embryonic expression pattern of Ubx-homologous transcripts
      coincides with the phenotypes associated with Utx mutations, providing support
      that the Ubx-homologous cloned DNA represents the Utx locus. By mid-germband
      extension Utx transcripts are expressed in a pattern similar to Ubx in
      Drosophila. However, during early germband formation Utx transcripts differ in
      both spatial and temporal progression. Utx expression is initially detected in
      parasegments 4 and 5 (T1p-T3a) as they are established during early germband
      formation. This is the first report of the wild-type parasegmental expression of 
      an insect Ubx ortholog extending through parasegment 4. The earlier and more
      anterior expression in the thorax may underlie the modification of the
      Coleopteran thorax.
FAU - Bennett, R L
AU  - Bennett RL
AD  - Zoology Department, Brigham Young University, Provo, UT 84602, USA.
      Randy_Bennett@byu.edu.
FAU - Brown, S J
AU  - Brown SJ
FAU - Denell, R E
AU  - Denell RE
LA  - eng
SI  - GENBANK/AF146649
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Dev Genes Evol
JT  - Development genes and evolution
JID - 9613264
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Insect Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Ubx protein, Drosophila)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/*genetics
MH  - Drosophila/genetics
MH  - *Drosophila Proteins
MH  - *Genes, Insect
MH  - Homeodomain Proteins/*genetics
MH  - Insect Proteins/genetics
MH  - Molecular Sequence Data
MH  - Sequence Homology
MH  - *Transcription Factors
MH  - Tribolium/*genetics
EDAT- 1999/11/07 00:00
MHDA- 1999/11/07 00:01
CRDT- 1999/11/07 00:00
PHST- 1999/11/07 00:00 [pubmed]
PHST- 1999/11/07 00:01 [medline]
PHST- 1999/11/07 00:00 [entrez]
AID - 92090608.427 [pii]
AID - 10.1007/s004270050295 [doi]
PST - ppublish
SO  - Dev Genes Evol. 1999 Oct;209(10):608-19. doi: 10.1007/s004270050295.