PMID- 10551863
OWN - NLM
STAT- MEDLINE
DCOM- 20000103
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 46
DP  - 1999 Nov 12
TI  - Mouse jagged1 physically interacts with notch2 and other notch receptors.
      Assessment by quantitative methods.
PG  - 32961-9
AB  - The Delta/Serrate/LAG-2 (DSL) domain containing proteins are considered to be
      ligands for Notch receptors. However, the physical interaction between DSL
      proteins and Notch receptors is poorly understood. In this study, we cloned a
      cDNA for mouse Jagged1 (mJagged1). To identify the receptor interacting with
      mJagged1 and to gain insight into its binding characteristics, we established two
      experimental systems using fusion proteins comprising various extracellular parts
      of mJagged1, a "cell" binding assay and a "solid-phase" binding assay. mJagged1
      physically bound to mouse Notch2 (mNotch2) on the cell surface and to a purified 
      extracellular portion of mNotch2, respectively, in a Ca(2+)-dependent manner.
      Scatchard analysis of mJagged1 binding to BaF3 cells and to the soluble Notch2
      protein demonstrated dissociation constants of 0.4 and 0.7 nM, respectively, and 
      that the number of mJagged1-binding sites on BaF3 is 5,548 per cell. Furthermore,
      deletion mutant analyses showed that the DSL domain of mJagged1 is a minimal
      binding unit and is indispensable for binding to mNotch2. The epidermal growth
      factor-like repeats of mJagged1 modulate the affinity of the interaction, with
      the first and second repeats playing a major role. Finally, solid-phase binding
      assay showed that Jagged1 binds to Notch1 and Notch3 in addition to Notch2,
      suggesting that mJagged1 is a ligand for multiple Notch receptors.
FAU - Shimizu, K
AU  - Shimizu K
AD  - Department of Hematology and Oncology, Graduate School of Medicine, University of
      Tokyo, Tokyo, 113-8655 Japan.
FAU - Chiba, S
AU  - Chiba S
FAU - Kumano, K
AU  - Kumano K
FAU - Hosoya, N
AU  - Hosoya N
FAU - Takahashi, T
AU  - Takahashi T
FAU - Kanda, Y
AU  - Kanda Y
FAU - Hamada, Y
AU  - Hamada Y
FAU - Yazaki, Y
AU  - Yazaki Y
FAU - Hirai, H
AU  - Hirai H
LA  - eng
SI  - GENBANK/AF171092
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Jag1 protein, mouse)
RN  - 0 (Jagged-1 Protein)
RN  - 0 (Membrane Proteins)
RN  - 0 (Notch1 protein, mouse)
RN  - 0 (Notch2 protein, mouse)
RN  - 0 (Notch3 protein, mouse)
RN  - 0 (Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptor, Notch1)
RN  - 0 (Receptor, Notch2)
RN  - 0 (Receptor, Notch3)
RN  - 0 (Receptor, Notch4)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Notch)
RN  - 0 (Serrate-Jagged Proteins)
RN  - 0 (Transcription Factors)
RN  - 146991-60-8 (Notch4 protein, mouse)
RN  - 62229-50-9 (Epidermal Growth Factor)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - CHO Cells
MH  - Calcium-Binding Proteins
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - Epidermal Growth Factor/chemistry
MH  - Intercellular Signaling Peptides and Proteins
MH  - Jagged-1 Protein
MH  - Membrane Proteins/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Protein Binding
MH  - Proteins/chemistry/*genetics
MH  - Proto-Oncogene Proteins/metabolism
MH  - RNA, Messenger/metabolism
MH  - Receptor, Notch1
MH  - Receptor, Notch2
MH  - Receptor, Notch3
MH  - Receptor, Notch4
MH  - Receptors, Cell Surface/*metabolism
MH  - Receptors, Notch
MH  - Sequence Homology, Amino Acid
MH  - Serrate-Jagged Proteins
MH  - *Transcription Factors
MH  - Transfection
EDAT- 1999/11/07 00:00
MHDA- 1999/11/07 00:01
CRDT- 1999/11/07 00:00
PHST- 1999/11/07 00:00 [pubmed]
PHST- 1999/11/07 00:01 [medline]
PHST- 1999/11/07 00:00 [entrez]
AID - 10.1074/jbc.274.46.32961 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Nov 12;274(46):32961-9. doi: 10.1074/jbc.274.46.32961.