PMID- 10551859
OWN - NLM
STAT- MEDLINE
DCOM- 20000103
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 46
DP  - 1999 Nov 12
TI  - Expression of BRC repeats in breast cancer cells disrupts the BRCA2-Rad51 complex 
      and leads to radiation hypersensitivity and loss of G(2)/M checkpoint control.
PG  - 32931-5
AB  - BRCA2 is a breast tumor suppressor with a potential function in the cellular 
      response to DNA damage. BRCA2 binds to Rad51 through its BRC repeats. In support 
      of the biological significance of this interaction, we found that the complex of 
      BRCA2 and Rad51 in breast cancer MCF-7 cells was diminished upon conditional 
      expression of a wild-type, but not a mutated, BRC4 repeat using the 
      tetracycline-inducible system. Cells expressing a wild-type BRC4 repeat showed 
      hypersensitivity to gamma-irradiation, an inability to form Rad51 
      radiation-induced foci, and a failure of radiation-induced G(2)/M, but not 
      G(1)/S, checkpoint control. These results strongly suggest that the interaction 
      between BRCA2 and Rad51 mediated by BRC repeats is critical for the cellular 
      response to DNA damage.
FAU - Chen, C F
AU  - Chen CF
AD  - Department of Molecular Medicine, Institute of Biotechnology, University of Texas 
      Health Science Center, San Antonio, Texas 78245, USA.
FAU - Chen, P L
AU  - Chen PL
FAU - Zhong, Q
AU  - Zhong Q
FAU - Sharp, Z D
AU  - Sharp ZD
FAU - Lee, W H
AU  - Lee WH
LA  - eng
GR  - CA30195/CA/NCI NIH HHS/United States
GR  - CA58183/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (BRCA2 Protein)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Transcription Factors)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 2.7.7.- (RAD51 protein, human)
RN  - EC 2.7.7.- (Rad51 Recombinase)
RN  - F8VB5M810T (Tetracycline)
SB  - IM
MH  - Amino Acid Sequence
MH  - BRCA2 Protein
MH  - Breast Neoplasms
MH  - Cell Cycle/*radiation effects
MH  - Clone Cells/radiation effects
MH  - DNA Damage/genetics
MH  - DNA-Binding Proteins/*genetics/metabolism
MH  - G2 Phase
MH  - Gamma Rays
MH  - Green Fluorescent Proteins
MH  - Humans
MH  - Luminescent Proteins
MH  - Microscopy, Fluorescence
MH  - Mitosis
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Neoplasm Proteins/*genetics/metabolism
MH  - Protein Binding
MH  - Rad51 Recombinase
MH  - Repetitive Sequences, Nucleic Acid/genetics
MH  - S Phase
MH  - Tetracycline/pharmacology
MH  - Transcription Factors/*genetics/metabolism
MH  - Tumor Cells, Cultured
EDAT- 1999/11/07 00:00
MHDA- 1999/11/07 00:01
CRDT- 1999/11/07 00:00
PHST- 1999/11/07 00:00 [pubmed]
PHST- 1999/11/07 00:01 [medline]
PHST- 1999/11/07 00:00 [entrez]
AID - S0021-9258(17)46598-7 [pii]
AID - 10.1074/jbc.274.46.32931 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Nov 12;274(46):32931-5. doi: 10.1074/jbc.274.46.32931.