PMID- 10551828
OWN - NLM
STAT- MEDLINE
DCOM- 20000103
LR  - 20191210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 46
DP  - 1999 Nov 12
TI  - Vaults and telomerase share a common subunit, TEP1.
PG  - 32712-7
AB  - Vaults are large cytoplasmic ribonucleoprotein complexes of undetermined
      function. Mammalian vaults have two high molecular mass proteins of 193 and 240
      kDa. We have identified a partial cDNA encoding the 240-kDa vault protein and
      determined it is identical to the mammalian telomerase-associated component,
      TEP1. TEP1 is the mammalian homolog of the Tetrahymena p80 telomerase protein and
      has been shown to interact specifically with mammalian telomerase RNA and the
      catalytic protein subunit hTERT. We show that while TEP1 is a component of the
      vault particle, vaults have no detectable telomerase activity. Using a yeast
      three-hybrid assay we demonstrate that several of the human vRNAs interact in a
      sequence-specific manner with TEP1. The presence of 16 WD40 repeats in the
      carboxyl terminus of the TEP1 protein is a convenient number for this protein to 
      serve a structural or organizing role in the vault, a particle with eight-fold
      symmetry. The sharing of the TEP1 protein between vaults and telomerase suggests 
      that TEP1 may play a common role in some aspect of ribonucleoprotein structure,
      function, or assembly.
FAU - Kickhoefer, V A
AU  - Kickhoefer VA
AD  - Department of Biological Chemistry, UCLA School of Medicine and Jonsson
      Comprehensive Cancer Center, Los Angeles, California 90095, USA.
      vkick@mednet.ucla.edu
FAU - Stephen, A G
AU  - Stephen AG
FAU - Harrington, L
AU  - Harrington L
FAU - Robinson, M O
AU  - Robinson MO
FAU - Rome, L H
AU  - Rome LH
LA  - eng
GR  - GM38097/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (TEP1 protein, human)
RN  - 0 (Tep1 protein, mouse)
RN  - 0 (Vault Ribonucleoprotein Particles)
RN  - 0 (telomerase RNA)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.7.49 (Telomerase)
RN  - EC 2.7.7.49 (telomerase p80 protein, Tetrahymena)
SB  - IM
MH  - Animals
MH  - Carrier Proteins/*chemistry
MH  - Centrifugation, Density Gradient
MH  - DNA-Binding Proteins
MH  - Haplorhini
MH  - HeLa Cells
MH  - Humans
MH  - Mice
MH  - Precipitin Tests
MH  - Protein Binding
MH  - RNA/metabolism
MH  - RNA, Messenger/analysis
MH  - RNA-Binding Proteins/chemistry/genetics
MH  - Telomerase/*chemistry/metabolism
MH  - Vault Ribonucleoprotein Particles/*chemistry/genetics
MH  - Yeasts
EDAT- 1999/11/07 00:00
MHDA- 1999/11/07 00:01
CRDT- 1999/11/07 00:00
PHST- 1999/11/07 00:00 [pubmed]
PHST- 1999/11/07 00:01 [medline]
PHST- 1999/11/07 00:00 [entrez]
AID - 10.1074/jbc.274.46.32712 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Nov 12;274(46):32712-7. doi: 10.1074/jbc.274.46.32712.