PMID- 10550324
OWN - NLM
STAT- MEDLINE
DCOM- 19991130
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 0002-9440 (Linking)
VI  - 155
IP  - 5
DP  - 1999 Nov
TI  - Nephrin localizes at the podocyte filtration slit area and is characteristically 
      spliced in the human kidney.
PG  - 1681-7
AB  - Defects in the newly reported gene NPHS1 in chromosome 19 cause the massive
      proteinuria of Finnish type congenital nephrotic syndrome (CNF). Together with
      its gene product, nephrin, NPHS1 is providing new understanding of the
      pathophysiological mechanisms of glomerular filtration. Here we show the
      characteristic splicing of NPHS1 mRNA in the normal and CNF kidneys and localize 
      nephrin exclusively in the glomerulus and to the filtration slit area by light
      and immunoelectron microscopy. These results indicate that nephrin is a new
      protein of the interpodocyte filtration slit area with a profound role in the
      pathophysiology of the filtration barrier.
FAU - Holthofer, H
AU  - Holthofer H
AD  - Division of Bacteriology, Haartman Institute, University of Helsinki, Helsinki,
      Finland. harry.holthofer@helsinki.fi
FAU - Ahola, H
AU  - Ahola H
FAU - Solin, M L
AU  - Solin ML
FAU - Wang, S
AU  - Wang S
FAU - Palmen, T
AU  - Palmen T
FAU - Luimula, P
AU  - Luimula P
FAU - Miettinen, A
AU  - Miettinen A
FAU - Kerjaschki, D
AU  - Kerjaschki D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Membrane Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (nephrin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Humans
MH  - Immunohistochemistry
MH  - Kidney Glomerulus/*metabolism/pathology/ultrastructure
MH  - Membrane Proteins
MH  - Microscopy, Immunoelectron
MH  - Molecular Sequence Data
MH  - Nephrotic Syndrome/*metabolism/pathology
MH  - Protein Isoforms/genetics/metabolism
MH  - Proteins/genetics/*metabolism
MH  - RNA Splicing
MH  - RNA, Messenger/analysis/genetics
PMC - PMC1866978
EDAT- 1999/11/07 00:00
MHDA- 1999/11/07 00:01
CRDT- 1999/11/07 00:00
PHST- 1999/11/07 00:00 [pubmed]
PHST- 1999/11/07 00:01 [medline]
PHST- 1999/11/07 00:00 [entrez]
AID - S0002-9440(10)65483-1 [pii]
AID - 10.1016/S0002-9440(10)65483-1 [doi]
PST - ppublish
SO  - Am J Pathol. 1999 Nov;155(5):1681-7. doi: 10.1016/S0002-9440(10)65483-1.