PMID- 10550218
OWN - NLM
STAT- MEDLINE
DCOM- 20000229
LR  - 20031114
IS  - 0896-8411 (Print)
IS  - 0896-8411 (Linking)
VI  - 13
IP  - 3
DP  - 1999 Nov
TI  - Expression and alternative splicing of the mouse autoimmune regulator gene
      (Aire).
PG  - 307-13
AB  - AIRE, the gene responsible for the autoimmune polyglandular syndrome type 1
      (APS1) or APECED, may act as a transcription factor according to its predicated
      protein structure. Here we demonstrate the low expression level of the mouse Aire
      gene as it is undetectable by Northern blot analyses. However, RT-PCR analyses
      revealed expression of Aire in mouse thymus, ovary, lung, testis, kidney and
      adrenal gland. Barely detectable level of RT-PCR product was also found in
      thyroid gland and heart but no amplification was detected in pancreas, spleen and
      liver. Competitive RT-PCR assays demonstrated highest expression level of Aire
      mRNA in thymus. In addition to the complete cDNA (Aire or Aire-1a), we identified
      11 alternative splicing forms (designated as Aire-1b, Aire-1c, Aire-1d, Aire-2a, 
      Aire-2b, Aire-2c, Aire-2d, Aire-3a, Aire-3b, Aire-3c and Aire-3d). These forms
      result from combinations of four alternative splicing units (exon 10, exon 11, 12
      bp in exon 6 and 3 bp in exon 8). The relative abundance of these splicing forms 
      was also determined.
CI  - Copyright 1999 Academic Press.
FAU - Ruan, Q G
AU  - Ruan QG
AD  - Department of Pathology, Immunology and Laboratory Medicine and Center for
      Mammalian Genetics, College of Medicine, University of Florida, Gainesville, FL
      32610, USA.
FAU - Wang, C Y
AU  - Wang CY
FAU - Shi, J D
AU  - Shi JD
FAU - She, J X
AU  - She JX
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Autoimmun
JT  - Journal of autoimmunity
JID - 8812164
RN  - 0 (APECED protein)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Alternative Splicing
MH  - Animals
MH  - Base Sequence
MH  - Exons
MH  - Gene Expression
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Polyendocrinopathies, Autoimmune/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Thymus Gland/metabolism
MH  - Tissue Distribution
MH  - Transcription Factors/biosynthesis/*genetics
EDAT- 1999/11/07 09:00
MHDA- 2000/03/04 09:00
CRDT- 1999/11/07 09:00
PHST- 1999/11/07 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 1999/11/07 09:00 [entrez]
AID - 10.1006/jaut.1999.0326 [doi]
AID - S0896-8411(99)90326-8 [pii]
PST - ppublish
SO  - J Autoimmun. 1999 Nov;13(3):307-13. doi: 10.1006/jaut.1999.0326.