PMID- 10550055
OWN - NLM
STAT- MEDLINE
DCOM- 19991210
LR  - 20190619
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 286
IP  - 5442
DP  - 1999 Nov 5
TI  - Requirement of ATM-dependent phosphorylation of brca1 in the DNA damage response 
      to double-strand breaks.
PG  - 1162-6
AB  - The Brca1 (breast cancer gene 1) tumor suppressor protein is phosphorylated in
      response to DNA damage. Results from this study indicate that the checkpoint
      protein kinase ATM (mutated in ataxia telangiectasia) was required for
      phosphorylation of Brca1 in response to ionizing radiation. ATM resides in a
      complex with Brca1 and phosphorylated Brca1 in vivo and in vitro in a region that
      contains clusters of serine-glutamine residues. Phosphorylation of this domain
      appears to be functionally important because a mutated Brca1 protein lacking two 
      phosphorylation sites failed to rescue the radiation hypersensitivity of a
      Brca1-deficient cell line. Thus, phosphorylation of Brca1 by the checkpoint
      kinase ATM may be critical for proper responses to DNA double-strand breaks and
      may provide a molecular explanation for the role of ATM in breast cancer.
FAU - Cortez, D
AU  - Cortez D
AD  - Verna and Mars McLean Department of Biochemistry and Molecular Biology, Howard
      Hughes Medical Institute, Department of Cell Biology, Baylor College of Medicine,
      Houston, TX 77030, USA.
FAU - Wang, Y
AU  - Wang Y
FAU - Qin, J
AU  - Qin J
FAU - Elledge, S J
AU  - Elledge SJ
LA  - eng
GR  - GM44664/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (BRCA1 Protein)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Tumor Suppressor Proteins)
RN  - EC 2.7.11.1 (ATM protein, human)
RN  - EC 2.7.11.1 (Ataxia Telangiectasia Mutated Proteins)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
SB  - IM
CIN - Science. 1999 Nov 5;286(5442):1100-2. PMID: 10610523
MH  - Amino Acid Sequence
MH  - Ataxia Telangiectasia/genetics
MH  - Ataxia Telangiectasia Mutated Proteins
MH  - BRCA1 Protein/*metabolism
MH  - Breast Neoplasms/genetics
MH  - Cell Cycle Proteins
MH  - Cell Line
MH  - *DNA Damage
MH  - *DNA Repair
MH  - DNA, Complementary
MH  - DNA-Binding Proteins
MH  - Female
MH  - Gamma Rays
MH  - Genes, BRCA1
MH  - Genetic Predisposition to Disease
MH  - HeLa Cells
MH  - Heterozygote
MH  - Humans
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Phosphorylation
MH  - Protein-Serine-Threonine Kinases/genetics/*metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Tumor Suppressor Proteins
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
AID - 7959 [pii]
AID - 10.1126/science.286.5442.1162 [doi]
PST - ppublish
SO  - Science. 1999 Nov 5;286(5442):1162-6. doi: 10.1126/science.286.5442.1162.