PMID- 10549853
OWN - NLM
STAT- MEDLINE
DCOM- 19991217
LR  - 20190719
IS  - 0918-6158 (Print)
IS  - 0918-6158 (Linking)
VI  - 22
IP  - 10
DP  - 1999 Oct
TI  - Cellular glutathione peroxidase as a predominant scavenger of
      hydroperoxyeicosatetraenoic acids in rabbit alveolar macrophages.
PG  - 1047-51
AB  - The cytosol of rabbit lung alveolar macrophages contains a high amount of
      peroxidase, which reduces 5-hydroperoxyeicosatetraenoic acid (5-HPETE) to
      5-hydroxyeicosatetraenoic acid (5-HETE) in the presence of glutathione. This
      peroxidase was purified 69-fold to homogeneity with overall recovery of activity 
      of 18.5%. The molecular mass of the enzyme was approximately 80 kDa by gel
      filtration, and emerged as a single band at 23.1 kDa under reducing condition by 
      sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The
      amino-terminal sequence of the purified peroxidase was completely identical to
      the sequence deduced from cellular glutathione peroxidase (cGPx) gene of rabbit
      liver. No other activity that reduces 5-HPETE to 5-HETE was observed during
      purification. These results suggest that cGPx plays an important role in
      metabolism of lipid hydroperoxides, especially HPETE, in lung alveolar
      macrophages.
FAU - Chiba, N
AU  - Chiba N
AD  - Japan Energy Research Center Co., Ltd., Tokyo.
FAU - Imai, H
AU  - Imai H
FAU - Narashima, K
AU  - Narashima K
FAU - Arai, M
AU  - Arai M
FAU - Oshima, G
AU  - Oshima G
FAU - Kunimoto, M
AU  - Kunimoto M
FAU - Nakagawa, Y
AU  - Nakagawa Y
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Leukotrienes)
RN  - 100896-35-3 (8-hydroperoxyeicosatetraenoic acid)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Glutathione Peroxidase/*metabolism
MH  - In Vitro Techniques
MH  - Leukotrienes/*metabolism
MH  - Macrophages, Alveolar/*enzymology/metabolism
MH  - Molecular Sequence Data
MH  - Rabbits
MH  - Sequence Analysis
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
AID - 10.1248/bpb.22.1047 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 1999 Oct;22(10):1047-51. doi: 10.1248/bpb.22.1047.