PMID- 10549626
OWN - NLM
STAT- MEDLINE
DCOM- 19991123
LR  - 20190915
IS  - 1074-7613 (Print)
IS  - 1074-7613 (Linking)
VI  - 11
IP  - 4
DP  - 1999 Oct
TI  - Differential roles of TLR2 and TLR4 in recognition of gram-negative and
      gram-positive bacterial cell wall components.
PG  - 443-51
AB  - Toll-like receptor (TLR) 2 and TLR4 are implicated in the recognition of various 
      bacterial cell wall components, such as lipopolysaccharide (LPS). To investigate 
      in vivo roles of TLR2, we generated TLR2-deficient mice. In contrast to LPS
      unresponsiveness in TLR4-deficient mice, TLR2-deficient mice responded to LPS to 
      the same extent as wild-type mice. TLR2-deficient macrophages were hyporesponsive
      to several Gram-positive bacterial cell walls as well as Staphylococcus aureus
      peptidoglycan. TLR4-deficient macrophages lacked the response to Gram-positive
      lipoteichoic acids. These results demonstrate that TLR2 and TLR4 recognize
      different bacterial cell wall components in vivo and TLR2 plays a major role in
      Gram-positive bacterial recognition.
FAU - Takeuchi, O
AU  - Takeuchi O
AD  - Department of Host Defense, Research Institute for Microbial Diseases, Osaka
      University, Japan.
FAU - Hoshino, K
AU  - Hoshino K
FAU - Kawai, T
AU  - Kawai T
FAU - Sanjo, H
AU  - Sanjo H
FAU - Takada, H
AU  - Takada H
FAU - Ogawa, T
AU  - Ogawa T
FAU - Takeda, K
AU  - Takeda K
FAU - Akira, S
AU  - Akira S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Lipid A)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Peptidoglycan)
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Teichoic Acids)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Toll-Like Receptors)
RN  - 56411-57-5 (lipoteichoic acid)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.1 (Interleukin-1 Receptor-Associated Kinases)
SB  - IM
MH  - Animals
MH  - Antigens, Bacterial/*immunology
MH  - Cell Wall/*immunology
MH  - Corynebacterium diphtheriae/chemistry/immunology
MH  - *Drosophila Proteins
MH  - Escherichia coli/chemistry/immunology
MH  - Female
MH  - Gram-Negative Bacteria/*chemistry/immunology
MH  - Gram-Positive Bacteria/*chemistry/immunology
MH  - Interleukin-1 Receptor-Associated Kinases
MH  - Lipid A/immunology
MH  - Lipopolysaccharides/*immunology
MH  - Macrophages, Peritoneal/immunology
MH  - Male
MH  - Membrane Glycoproteins/deficiency/genetics/*physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - NF-kappa B/metabolism
MH  - Nocardia/chemistry/immunology
MH  - Peptidoglycan/*immunology
MH  - Protein Isoforms/deficiency/genetics/*physiology
MH  - Protein Kinases/metabolism
MH  - Receptors, Cell Surface/deficiency/genetics/*physiology
MH  - Salmonella/chemistry/immunology
MH  - Signal Transduction
MH  - Species Specificity
MH  - Staphylococcus aureus/chemistry/immunology
MH  - Teichoic Acids/*immunology
MH  - Toll-Like Receptor 2
MH  - Toll-Like Receptor 4
MH  - Toll-Like Receptors
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
AID - S1074-7613(00)80119-3 [pii]
AID - 10.1016/s1074-7613(00)80119-3 [doi]
PST - ppublish
SO  - Immunity. 1999 Oct;11(4):443-51. doi: 10.1016/s1074-7613(00)80119-3.