PMID- 10549285
OWN - NLM
STAT- MEDLINE
DCOM- 19991126
LR  - 20211203
IS  - 1097-2765 (Print)
IS  - 1097-2765 (Linking)
VI  - 4
IP  - 4
DP  - 1999 Oct
TI  - Heterochromatin dynamics in mouse cells: interaction between chromatin assembly
      factor 1 and HP1 proteins.
PG  - 529-40
AB  - Mechanisms contributing to the maintenance of heterochromatin in proliferating
      cells are poorly understood. We demonstrate that chromatin assembly factor 1
      (CAF-1) binds to mouse HP1 proteins via an N-terminal domain of its p150 subunit,
      a domain dispensable for nucleosome assembly during DNA replication. Mutations in
      p150 prevent association with HP1 in heterochromatin in cells that are not in S
      phase and the formation of CAF-1-HP1 complexes in nascent chromatin during DNA
      replication in vitro. We suggest that CAF-1 p150 has a heterochromatin-specific
      function distinct from its nucleosome assembly function during S phase. Just
      before mitosis, CAF-1 p150 and some HP1 progressively dissociate from
      heterochromatin concomitant with histone H3 phosphorylation. The HP1 proteins
      reassociate with chromatin at the end of mitosis, as histone H3 is
      dephosphorylated.
FAU - Murzina, N
AU  - Murzina N
AD  - Cambridge Centre for Molecular Recognition, Department of Biochemistry,
      University of Cambridge, United Kingdom.
FAU - Verreault, A
AU  - Verreault A
FAU - Laue, E
AU  - Laue E
FAU - Stillman, B
AU  - Stillman B
LA  - eng
SI  - GENBANK/AJ132771
GR  - CA13106/CA/NCI NIH HHS/United States
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell
JT  - Molecular cell
JID - 9802571
RN  - 0 (Chaf1a protein, mouse)
RN  - 0 (Chromatin Assembly Factor-1)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Heterochromatin)
RN  - 0 (Histones)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - 107283-02-3 (Chromobox Protein Homolog 5)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 2.3.2.27 (Trim28 protein, mouse)
RN  - EC 2.3.2.27 (Tripartite Motif-Containing Protein 28)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Chromatin Assembly Factor-1
MH  - Chromobox Protein Homolog 5
MH  - Chromosomal Proteins, Non-Histone/*metabolism
MH  - DNA Replication/genetics
MH  - DNA-Binding Proteins/*metabolism
MH  - Fluorescent Antibody Technique
MH  - Green Fluorescent Proteins
MH  - Heterochromatin/*metabolism
MH  - Histones/metabolism
MH  - Luminescent Proteins
MH  - Mice
MH  - Mitosis
MH  - Molecular Sequence Data
MH  - Mutation
MH  - *Nuclear Proteins
MH  - Phosphorylation
MH  - Protein Binding
MH  - Repressor Proteins/metabolism
MH  - S Phase
MH  - *Transcription Factors
MH  - Tripartite Motif-Containing Protein 28
MH  - Yeasts/genetics
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
AID - S1097-2765(00)80204-X [pii]
AID - 10.1016/s1097-2765(00)80204-x [doi]
PST - ppublish
SO  - Mol Cell. 1999 Oct;4(4):529-40. doi: 10.1016/s1097-2765(00)80204-x.