PMID- 10549282
OWN - NLM
STAT- MEDLINE
DCOM- 19991126
LR  - 20190915
IS  - 1097-2765 (Print)
IS  - 1097-2765 (Linking)
VI  - 4
IP  - 4
DP  - 1999 Oct
TI  - Interaction of the Ski oncoprotein with Smad3 regulates TGF-beta signaling.
PG  - 499-509
AB  - TGF-beta treatment of cells induces a variety of physiologic responses, including
      growth inhibition, differentiation, and induction of apoptosis. TGF-beta induces 
      phosphorylation and nuclear translocation of Smad3. We describe here the
      association of Smad3 with the nuclear protooncogene protein Ski in response to
      the activation of TGF-beta signaling. Association with Ski represses
      transcriptional activation by Smad3, and overexpression of Ski renders cells
      resistant to the growth-inhibitory effects of TGF-beta. The transcriptional
      repression as well as the growth resistance to TGF-beta by overexpression of Ski 
      can be overcome by overexpression of Smad3. These results demonstrate that Ski is
      a novel component of the TGF-beta signaling pathway and shed light on the
      mechanism of action of the Ski oncoprotein.
FAU - Sun, Y
AU  - Sun Y
AD  - Whitehead Institute for Biomedical Research, Nine Cambridge Center, Massachusetts
      02142, USA.
FAU - Liu, X
AU  - Liu X
FAU - Eaton, E N
AU  - Eaton EN
FAU - Lane, W S
AU  - Lane WS
FAU - Lodish, H F
AU  - Lodish HF
FAU - Weinberg, R A
AU  - Weinberg RA
LA  - eng
GR  - CA63260/CA/NCI NIH HHS/United States
GR  - R35CA39826/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell
JT  - Molecular cell
JID - 9802571
RN  - 0 (CDKN2B protein, human)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p15)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (SMAD3 protein, human)
RN  - 0 (Smad3 Protein)
RN  - 0 (Trans-Activators)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 126648-96-2 (SKI protein, human)
SB  - IM
MH  - Animals
MH  - Carrier Proteins/genetics
MH  - *Cell Cycle Proteins
MH  - Cell Division/genetics
MH  - Cell Nucleus/metabolism
MH  - Cells, Cultured
MH  - Cyclin-Dependent Kinase Inhibitor p15
MH  - *Cyclin-Dependent Kinase Inhibitor p16
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Gene Expression Regulation
MH  - Genes, myc/genetics
MH  - Humans
MH  - Mink
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - Proto-Oncogene Proteins/genetics/*metabolism
MH  - Repressor Proteins/genetics
MH  - Signal Transduction
MH  - Smad3 Protein
MH  - Trans-Activators/genetics/*metabolism
MH  - Transforming Growth Factor beta/*pharmacology
MH  - *Tumor Suppressor Proteins
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
AID - S1097-2765(00)80201-4 [pii]
AID - 10.1016/s1097-2765(00)80201-4 [doi]
PST - ppublish
SO  - Mol Cell. 1999 Oct;4(4):499-509. doi: 10.1016/s1097-2765(00)80201-4.