PMID- 10548734
OWN - NLM
STAT- MEDLINE
DCOM- 19991209
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 239
IP  - 2
DP  - 1999 Nov 1
TI  - Isolation and mapping of a human lung-specific gene, TSA1902, encoding a novel
      chitinase family member.
PG  - 325-31
AB  - Using differential display technique, we have isolated a novel human gene
      expressed specifically in the lung. Two forms of the gene, designated TSA1902,
      were transcribed by alternate mRNA splicing. The transcribed mRNAs, termed
      TSA1902-L and TSA1902-S, putatively encode proteins of 368 and 315 amino acids,
      respectively, which show high similarity to human chitotriosidase protein. The
      N-terminal region of TSA1902-L protein contains the conserved active site
      residues (DXXDXDXE) of the catalytic center of various chitinases which are
      essential for chitinase activity. The deduced protein sequence of TSA1902-S,
      however, does not possess this active site, with the N-terminal 54 amino acids
      present in TSA1902-L protein having been deleted. Both proteins lacked the
      secretory sequence of N-termini and, judging from the hydropathy profile, may be 
      soluble proteins in the cytoplasm. Chromosomal mapping by radiation hybrid
      analysis localized this gene to the chromosome 1p13.1-p21.3.
FAU - Saito, A
AU  - Saito A
AD  - Otsuka GEN Research Institute, Otsuka Pharmaceutical Co., Ltd., 463-10 Kagasuno
      Kawauchi-cho, Tokushima, Japan.
FAU - Ozaki, K
AU  - Ozaki K
FAU - Fujiwara, T
AU  - Fujiwara T
FAU - Nakamura, Y
AU  - Nakamura Y
FAU - Tanigami, A
AU  - Tanigami A
LA  - eng
SI  - GENBANK/AB025008
SI  - GENBANK/AB025009
PT  - Journal Article
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (DNA, Complementary)
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - EC 3.2.1.14 (CHIA protein, human)
RN  - EC 3.2.1.14 (Chitinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Chitinases/*genetics
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 1/genetics
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry/genetics/isolation & purification
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Isoenzymes/genetics
MH  - Lung/*enzymology
MH  - Male
MH  - Molecular Sequence Data
MH  - RNA, Messenger/genetics/metabolism
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
AID - S0378111999003947 [pii]
AID - 10.1016/s0378-1119(99)00394-7 [doi]
PST - ppublish
SO  - Gene. 1999 Nov 1;239(2):325-31. doi: 10.1016/s0378-1119(99)00394-7.