PMID- 10548543
OWN - NLM
STAT- MEDLINE
DCOM- 20000127
LR  - 20181113
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 344 Pt 1
DP  - 1999 Nov 15
TI  - Identification and molecular characterization of acyl-CoA synthetase in human
      erythrocytes and erythroid precursors.
PG  - 135-43
AB  - Full-length cDNA species encoding two forms of acyl-CoA synthetase from a K-562
      human erythroleukaemic cell line were cloned, sequenced and expressed. The first 
      form, named long-chain acyl-CoA synthetase 5 (LACS5), was found to be a novel,
      unreported, human acyl-CoA synthetase with high similarity to rat brain ACS2 (91%
      identical). The second form (66% identical with LACS5) was 97% identical with
      human liver LACS1. The LACS5 gene encodes a highly expressed 2.9 kb mRNA
      transcript in human haemopoietic stem cells from cord blood, bone marrow,
      reticulocytes and fetal blood cells derived from fetal liver. An additional 6.3
      kb transcript is also found in these erythrocyte precursors; 2.9 and 9.6 kb
      transcripts of LACS5 are found in human brain, but transcripts are virtually
      absent from human heart, kidney, liver, lung, pancreas, spleen and skeletal
      muscle. The 78 kDa expressed LACS5 protein used the long-chain fatty acids
      palmitic acid, oleic acid and arachidonic acid as substrates. Antibodies directed
      against LACS5 cross-reacted with erythrocyte membranes. We conclude that early
      erythrocyte precursors express at least two different forms of acyl-CoA
      synthetase and that LACS5 is present in mature erythrocyte plasma membranes.
FAU - Malhotra, K T
AU  - Malhotra KT
AD  - Children's Hospital, Oakland Research Institute, 5700 Martin Luther King Jr. Way,
      Oakland, CA 94609, USA.
FAU - Malhotra, K
AU  - Malhotra K
FAU - Lubin, B H
AU  - Lubin BH
FAU - Kuypers, F A
AU  - Kuypers FA
LA  - eng
SI  - GENBANK/AF129166
GR  - DK 32094/DK/NIDDK NIH HHS/United States
GR  - HL27059/HL/NHLBI NIH HHS/United States
GR  - MO1RR01271/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - EC 6.2.1.- (Coenzyme A Ligases)
SB  - IM
MH  - Adult
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Coenzyme A Ligases/*blood/chemistry/genetics
MH  - DNA Primers/genetics
MH  - DNA, Complementary/genetics
MH  - Erythrocyte Membrane/enzymology
MH  - Erythrocytes/*enzymology
MH  - Fetal Blood/cytology/enzymology
MH  - Hematopoietic Stem Cells/*enzymology
MH  - Humans
MH  - In Vitro Techniques
MH  - Infant, Newborn
MH  - K562 Cells
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - RNA, Messenger/blood/genetics/metabolism
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
PMC - PMC1220623
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
PST - ppublish
SO  - Biochem J. 1999 Nov 15;344 Pt 1:135-43.