PMID- 10548495
OWN - NLM
STAT- MEDLINE
DCOM- 19991217
LR  - 20041118
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 265
IP  - 1
DP  - 1999 Nov
TI  - Cloning and expression of pleckstrin 2, a novel member of the pleckstrin family.
PG  - 87-93
AB  - A novel member of the pleckstrin family has been identified and designated as
      mouse pleckstrin 2. The cDNA clone with an insert of 1588 bp contained a 1059-bp 
      open reading frame encoding a polypeptide of 353 amino acid residues. The deduced
      amino acid sequence predicted that pleckstrin 2 has two pleckstrin homology (PH) 
      domains at the N- and C-termini and a DEP (Dsh, egl-10, and pleckstrin) domain in
      the central region and showed 35% identity with the sequence of chicken
      pleckstrin. Northern blot and reverse-transcription polymerase chain reaction
      analysis revealed that pleckstrin 2 mRNA is ubiquitously expressed. Southern blot
      analysis indicated that the mouse pleckstrin 2 gene may consisit of two or more
      exons. To obtain information relative to natural ligand(s) for each of the PH
      domains in vivo, we employed the green fluorescent protein (GFP) tagged fusion
      protein system. Distributions of N-terminal and C-terminal PH domains of
      pleckstrin 2 were quite different from each other, suggesting that these PH
      domains may interact with distinct factor(s).
CI  - Copyright 1999 Academic Press.
FAU - Inazu, T
AU  - Inazu T
AD  - Department of Biochemistry, Fukui Medical University, Matsuoka, Fukui, 910-1193, 
      Japan. tinazu@fmsrsa.fukui-med.ac.jp
FAU - Yamada, K
AU  - Yamada K
FAU - Miyamoto, K
AU  - Miyamoto K
LA  - eng
SI  - GENBANK/AF170564
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA Primers)
RN  - 0 (Membrane Proteins)
RN  - 0 (PLEK2 protein, human)
RN  - 0 (Plek2 protein, mouse)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chickens
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - Humans
MH  - Liver/*metabolism
MH  - Membrane Proteins/biosynthesis/*chemistry/*genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Organ Specificity
MH  - Recombinant Proteins/biosynthesis/chemistry
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Tumor Cells, Cultured
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
AID - 10.1006/bbrc.1999.1461 [doi]
AID - S0006-291X(99)91461-4 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Nov;265(1):87-93. doi: 10.1006/bbrc.1999.1461.