PMID- 10548487
OWN - NLM
STAT- MEDLINE
DCOM- 19991217
LR  - 20161124
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 265
IP  - 1
DP  - 1999 Nov
TI  - nRap GEP: a novel neural GDP/GTP exchange protein for rap1 small G protein that
      interacts with synaptic scaffolding molecule (S-SCAM).
PG  - 38-44
AB  - Synaptic scaffolding molecule (S-SCAM) has six PDZ domains through which it
      interacts with N-methyl-d-aspartate receptors and neuroligin at synaptic
      junctions. We isolated here a novel S-SCAM-binding protein. This protein has one 
      PDZ, one Ras association, one Ras GDP/GTP exchange protein (Ras GEP) domain, and 
      one C-terminal consensus motif for binding to PDZ domains. We named it nRap GEP
      (neural Rap GEP). nRap GEP moreover has an incomplete cyclic AMP (cAMP)-binding
      (CAB) domain. The domain organization of nRap GEP is similar to that of
      Epac/cAMP-guanine nucleotide exchange factor (GEF) I, except that Epac/cAMP-GEFI 
      has complete CAB and Ras GEP domains but lacks the other two domains and the
      C-terminal motif. nRap GEP showed GEP activity for Rap1 but did not bind cAMP.
      nRap GEP was specifically expressed in rat brain. Immunohistochemical analysis
      revealed that nRap GEP and S-SCAM were localized at synaptic areas of the
      cerebellum. These results suggest that nRap GEP is a novel neural Rap1-specific
      GEP which is associated with S-SCAM.
CI  - Copyright 1999 Academic Press.
FAU - Ohtsuka, T
AU  - Ohtsuka T
AD  - Department of Molecular Biology and Biochemistry, Osaka University Graduate
      School of Medicine/Faculty of Medicine, Suita, 565-0871, Japan.
FAU - Hata, Y
AU  - Hata Y
FAU - Ide, N
AU  - Ide N
FAU - Yasuda, T
AU  - Yasuda T
FAU - Inoue, E
AU  - Inoue E
FAU - Inoue, T
AU  - Inoue T
FAU - Mizoguchi, A
AU  - Mizoguchi A
FAU - Takai, Y
AU  - Takai Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (Guanine Nucleotide Exchange Factors)
RN  - 0 (Magi2 protein, rat)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RAPGEF2 protein, human)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.4.8 (Guanylate Kinases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - Brain/*metabolism
MH  - Carrier Proteins/*chemistry/*metabolism
MH  - Cloning, Molecular
MH  - GTP-Binding Proteins/*chemistry/genetics/*metabolism
MH  - *Guanine Nucleotide Exchange Factors
MH  - Guanylate Kinases
MH  - Humans
MH  - Kinetics
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/*chemistry/*metabolism
MH  - Rats
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Subcellular Fractions/metabolism
MH  - Synapses/*metabolism
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
AID - 10.1006/bbrc.1999.1619 [doi]
AID - S0006-291X(99)91619-4 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Nov;265(1):38-44. doi: 10.1006/bbrc.1999.1619.