PMID- 10548469
OWN - NLM
STAT- MEDLINE
DCOM- 19991227
LR  - 20161124
IS  - 0014-4835 (Print)
IS  - 0014-4835 (Linking)
VI  - 69
IP  - 5
DP  - 1999 Nov
TI  - Photoreceptor localization of the KIF3A and KIF3B subunits of the heterotrimeric 
      microtubule motor kinesin II in vertebrate retina.
PG  - 491-503
AB  - The heterotrimeric microtubule motor kinesin II has been shown to be required for
      morphogenesis and maintenance of both motile flagella and immotile sensory cilia.
      Recently, we showed that the KIF3A subunit of kinesin II is concentrated in the
      inner segment and connecting cilium of fish photoreceptors. Here we report the
      gene structure of human KIF3A (HsKIF3A) and describe its localization in human
      and monkey retina. We also describe the localization of both KIF3A and KIF3B
      kinesin II subunits in Xenopus retina. Using a portion of HsKIF3A we had
      amplified from adult human retinal cDNA, we found by a GenBank database search
      that an identical sequence had already been obtained by the Human Genome Center
      at Lawrence Berkeley National Laboratories in a direct sequencing analysis of 680
      kb of human chromosome 5q31. By comparing the genomic sequence of HsKIF3A to the 
      open reading frame (ORF) of the highly homologous mouse Kif3A, we determined that
      the HsKIF3A gene has 17 exons and an ORF of approximately 2.1 kb, predicting a
      protein of 80.3 kDa. Antibodies against sea urchin KRP85, a KIF3A homologue,
      bound to a single band of approximately 85 kDa in immunoblots of total retina
      protein from human, monkey and Xenopus. In these same samples, a single band of
      approximately 95 kDa is recognized by antibodies against Xklp3, a Xenopus KIF3B
      homologue. In sections of Xenopus retina, both antibodies strongly labelled
      photoreceptor inner segments and the outer limiting membrane. Both antibodies
      also labelled photoreceptor axonemes. The axonemal localization of kinesin II
      subunits suggests that kinesin II may play a role in transport of materials from 
      the photoreceptor cell body to the outer segment.
CI  - Copyright 1999 Academic Press.
FAU - Whitehead, J L
AU  - Whitehead JL
AD  - Department of Molecular and Cell Biology, University of California, 335 Life
      Sciences Addition, Berkeley, CA, 94720-3200, USA.
FAU - Wang, S Y
AU  - Wang SY
FAU - Bost-Usinger, L
AU  - Bost-Usinger L
FAU - Hoang, E
AU  - Hoang E
FAU - Frazer, K A
AU  - Frazer KA
FAU - Burnside, B
AU  - Burnside B
LA  - eng
GR  - EY03575/EY/NEI NIH HHS/United States
GR  - EY6534-02/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
RN  - 0 (KIF3A protein, human)
RN  - 0 (KIF3B protein, human)
RN  - 0 (Kif3a protein, mouse)
RN  - 0 (Kif3b protein, mouse)
RN  - EC 3.6.4.4 (Kinesin)
SB  - IM
MH  - Adult
MH  - Amino Acid Sequence
MH  - Animals
MH  - Exons
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Introns
MH  - Kinesin/*analysis/genetics
MH  - Macaca mulatta/*metabolism
MH  - Microscopy, Confocal
MH  - Molecular Sequence Data
MH  - Photoreceptor Cells, Vertebrate/*chemistry
MH  - Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Species Specificity
MH  - Xenopus laevis/*metabolism
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
AID - 10.1006/exer.1999.0724 [doi]
AID - S0014-4835(99)90724-8 [pii]
PST - ppublish
SO  - Exp Eye Res. 1999 Nov;69(5):491-503. doi: 10.1006/exer.1999.0724.