PMID- 10548110
OWN - NLM
STAT- MEDLINE
DCOM- 19991116
LR  - 20161124
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 401
IP  - 6755
DP  - 1999 Oct 21
TI  - Accumulation of cyclin B1 requires E2F and cyclin-A-dependent rearrangement of
      the anaphase-promoting complex.
PG  - 815-8
AB  - In mammalian somatic-cell cycles, progression through the G1-phase restriction
      point and initiation of DNA replication are controlled by the ability of the
      retinoblastoma tumour-suppressor protein (pRb) family to regulate the E2F/DP
      transcription factors. Continuing transcription of E2F target genes beyond the
      G1/S transition is required for coordinating S-phase progression with cell
      division, a process driven by cyclin-B-dependent kinase and anaphase-promoting
      complex (APC)-mediated proteolysis. How E2F-dependent events at G1/S transition
      are orchestrated with cyclin B and APC activity remains unknown. Here, using an
      in vivo assay to measure protein stability in real time during the cell cycle, we
      show that repression of E2F activity or inhibition of cyclin-A-dependent kinase
      in S phase triggers the destruction of cyclin B1 through the re-assembly of APC, 
      the ubiquitin ligase that is essential for mitotic cyclin proteolysis, with its
      activatory subunit Cdh1. Phosphorylation-deficient mutant Cdh1 or immunodepletion
      of cyclin A resulted in assembly of active Cdh1-APC even in S-phase cells. These 
      results implicate an E2F-dependent, cyclin A/Cdk2-mediated phosphorylation of
      Cdh1 in the timely accumulation of cyclin B1 and the coordination of cell-cycle
      progression during the post-restriction point period.
FAU - Lukas, C
AU  - Lukas C
AD  - Institute of Cancer Biology, Danish Cancer Society, Copenhagen.
FAU - Sorensen, C S
AU  - Sorensen CS
FAU - Kramer, E
AU  - Kramer E
FAU - Santoni-Rugiu, E
AU  - Santoni-Rugiu E
FAU - Lindeneg, C
AU  - Lindeneg C
FAU - Peters, J M
AU  - Peters JM
FAU - Bartek, J
AU  - Bartek J
FAU - Lukas, J
AU  - Lukas J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (CCNB1 protein, human)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cyclin A)
RN  - 0 (Cyclin B)
RN  - 0 (Cyclin B1)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (E2F Transcription Factors)
RN  - 0 (Retinoblastoma Protein)
RN  - 0 (Retinoblastoma-Binding Protein 1)
RN  - 0 (Transcription Factor DP1)
RN  - 0 (Transcription Factors)
RN  - EC 2.3.2.23 (Ubiquitin-Protein Ligase Complexes)
RN  - EC 2.3.2.27 (Anaphase-Promoting Complex-Cyclosome)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 6.- (Ligases)
SB  - IM
MH  - Anaphase/*physiology
MH  - Anaphase-Promoting Complex-Cyclosome
MH  - *Carrier Proteins
MH  - Cell Cycle/physiology
MH  - Cell Cycle Proteins/*metabolism
MH  - Cell Line
MH  - Cyclin A/*metabolism
MH  - Cyclin B/*metabolism
MH  - Cyclin B1
MH  - *DNA-Binding Proteins
MH  - E2F Transcription Factors
MH  - Humans
MH  - Ligases/*metabolism
MH  - Phosphorylation
MH  - Retinoblastoma Protein/metabolism
MH  - Retinoblastoma-Binding Protein 1
MH  - S Phase
MH  - Transcription Factor DP1
MH  - Transcription Factors/*metabolism
MH  - *Ubiquitin-Protein Ligase Complexes
MH  - Ubiquitin-Protein Ligases
EDAT- 1999/11/05 08:00
MHDA- 2001/03/23 10:01
CRDT- 1999/11/05 08:00
PHST- 1999/11/05 08:00 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/11/05 08:00 [entrez]
AID - 10.1038/44611 [doi]
PST - ppublish
SO  - Nature. 1999 Oct 21;401(6755):815-8. doi: 10.1038/44611.