PMID- 10545525
OWN - NLM
STAT- MEDLINE
DCOM- 19991207
LR  - 20181113
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 104
IP  - 9
DP  - 1999 Nov
TI  - Defining a novel 75-kDa phosphoprotein associated with SS-A/Ro and identification
      of distinct human autoantibodies.
PG  - 1265-75
AB  - Mothers of children with neonatal lupus erythematosus (NLE) and heart block, as
      well as patients with Sjogren's syndrome (SS) and systemic lupus erythematosus,
      have serum autoantibodies that recognize SS-A/Ro autoantigens including the
      60-kDa ribonucleoprotein. By yeast 2-hybrid screening, we identified a novel
      75-kDa protein (pp75) that interacts with the carboxyl 70% of 60-kDa SS-A/Ro. The
      specificity of interaction was confirmed using mammalian 2-hybrid and chemical
      crosslinking studies. Immunoprecipitation with radiolabeled HeLa cell extracts
      showed that pp75 was phosphorylated and associated with 2 other phosphoproteins
      of 64 kDa. In Northern blot analysis, pp75 was expressed in all tissues analyzed;
      the highest expression was in the human heart. Based on immunofluorescence of
      transfected HeLa cells, pp75 is localized predominantly in the cytoplasm, an
      observation confirmed by immunohistochemistry in untransfected cells. Based on
      Western blot and ELISA assays, sera from 14 of 84 mothers of children with NLE
      recognized pp75, including 1 mother in whom anti-SS-A/Ro antibodies were not
      detected. In addition, sera from 5 of 80 patients with SS were positive for
      anti-pp75 antibody. Identification of molecular partners is a first step toward
      elucidating the functions and possible involvement in pathogenesis of
      long-recognized autoantigens such as 60-kDa SS-A/Ro, which are at present poorly 
      understood.
FAU - Wang, D
AU  - Wang D
AD  - W.M. Keck Autoimmune Disease Center, DNA Core Laboratory for Structural Analysis,
      Department of Molecular Medicine, The Scripps Research Institute, La Jolla,
      California 92037, USA.
FAU - Buyon, J P
AU  - Buyon JP
FAU - Zhu, W
AU  - Zhu W
FAU - Chan, E K
AU  - Chan EK
LA  - eng
SI  - GENBANK/AF153085
GR  - AR-42455/AR/NIAMS NIH HHS/United States
GR  - N01AR42220/AR/NIAMS NIH HHS/United States
GR  - M01 RR000833/RR/NCRR NIH HHS/United States
GR  - R01 AR042455/AR/NIAMS NIH HHS/United States
GR  - R37 AR042455/AR/NIAMS NIH HHS/United States
GR  - AR-41803/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Autoantibodies)
RN  - 0 (Autoantigens)
RN  - 0 (Carrier Proteins)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (RAB11FIP5 protein, human)
RN  - 0 (RNA, Small Cytoplasmic)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (SS-A antigen)
RN  - 0 (TROVE2 protein, human)
RN  - 0 (phosphoprotein, 75-kDa)
SB  - AIM
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Autoantibodies/*immunology
MH  - Autoantigens/*immunology
MH  - Base Sequence
MH  - Blotting, Western
MH  - COS Cells
MH  - *Carrier Proteins
MH  - Cloning, Molecular
MH  - Female
MH  - HeLa Cells
MH  - Humans
MH  - Lupus Erythematosus, Systemic/congenital/immunology
MH  - Maternal-Fetal Exchange
MH  - *Mitochondrial Proteins
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - Myocardium/metabolism
MH  - Phosphoproteins/*chemistry/*genetics/immunology
MH  - Precipitin Tests
MH  - Pregnancy
MH  - *RNA, Small Cytoplasmic
MH  - Ribonucleoproteins/*immunology
MH  - Sjogren's Syndrome/congenital/immunology
MH  - Tissue Distribution
MH  - Transfection
MH  - Two-Hybrid System Techniques
PMC - PMC409828
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
AID - 10.1172/JCI8003 [doi]
PST - ppublish
SO  - J Clin Invest. 1999 Nov;104(9):1265-75. doi: 10.1172/JCI8003.