PMID- 10545197
OWN - NLM
STAT- MEDLINE
DCOM- 19991222
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 38
IP  - 44
DP  - 1999 Nov 2
TI  - Molecular association between ATR and two components of the nucleosome remodeling
      and deacetylating complex, HDAC2 and CHD4.
PG  - 14711-7
AB  - Ataxia telangiectasia mutated (ATM)- and Rad3-related protein (ATR) is a
      phosphatidylinositol-kinase (PIK)-related kinase that has been implicated in the 
      response of human cells to multiple forms of DNA damage and may play a role in
      the DNA replication checkpoint. The purification of an ATR complex allowed
      identification of chromodomain-helicase-DNA-binding protein 4 (CHD4) as an
      ATR-associated protein by tandem mass spectrometric sequencing. CHD4 (also called
      Mi-2beta) is a component of a histone-deacetylase-2 (HDAC2)-containing complex,
      the nucleosome remodeling and deacetylating (NRD) complex. Endogenous ATR, CHD4, 
      and HDAC2 are shown to coimmunoprecipitate, and ATR and HDAC2 coelute through two
      biochemical purification steps. Other members of the NRD complex, HDAC1, MTA1,
      and MTA2, are also detectable in ATR immunoprecipitates. ATR's association with
      CHD4 and HDAC2 suggests that there may be a linkage between ATR's role in
      mediating checkpoints induced by DNA damage and chromatin modulation via
      remodeling and deacetylation.
FAU - Schmidt, D R
AU  - Schmidt DR
AD  - Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology,
      Harvard University, Cambridge, Massachusetts 02138, USA.
      schmidt@slsiris.harvard.edu
FAU - Schreiber, S L
AU  - Schreiber SL
LA  - eng
GR  - GM-52067/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Autoantigens)
RN  - 0 (CHD4 protein, human)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Nucleosomes)
RN  - 0 (Repressor Proteins)
RN  - EC 2.7.11.1 (ATR protein, human)
RN  - EC 2.7.11.1 (Ataxia Telangiectasia Mutated Proteins)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 3.5.1.98 (Histone Deacetylase 2)
RN  - EC 3.5.1.98 (Histone Deacetylases)
RN  - EC 3.5.1.98 (Mi-2 Nucleosome Remodeling and Deacetylase Complex)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.4.- (DNA Helicases)
SB  - IM
MH  - Acetylation
MH  - Adenosine Triphosphatases/*chemistry/isolation & purification/*metabolism
MH  - Amino Acid Sequence
MH  - Ataxia Telangiectasia Mutated Proteins
MH  - *Autoantigens
MH  - Cell Cycle
MH  - Cell Cycle Proteins/*chemistry/isolation & purification/*metabolism
MH  - Cell Line
MH  - DNA Damage
MH  - DNA Helicases/*chemistry/isolation & purification/*metabolism
MH  - DNA Replication
MH  - HeLa Cells
MH  - Histone Deacetylase 2
MH  - Histone Deacetylases/*chemistry/isolation & purification/*metabolism
MH  - Humans
MH  - Macromolecular Substances
MH  - Mi-2 Nucleosome Remodeling and Deacetylase Complex
MH  - Nucleosomes/*metabolism
MH  - *Protein-Serine-Threonine Kinases
MH  - *Repressor Proteins
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
AID - bi991614n [pii]
AID - 10.1021/bi991614n [doi]
PST - ppublish
SO  - Biochemistry. 1999 Nov 2;38(44):14711-7. doi: 10.1021/bi991614n.