PMID- 10545110
OWN - NLM
STAT- MEDLINE
DCOM- 20000104
LR  - 20191210
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 21
DP  - 1999 Nov 1
TI  - Ca(2+)-ATPase function is required for intracellular trafficking of the Notch
      receptor in Drosophila.
PG  - 5983-93
AB  - Maintaining high Ca(2+) concentrations in the lumen of the endoplasmic reticulum 
      is important for protein synthesis and transport. We identified a lethal
      complementation group recovered in a screen for mutations that reduce Notch
      activity as loss-of-function alleles of the Drosophila Ca(2+)-ATPase gene
      Ca-P60A. Analysis of Ca-P60A mutants indicates that Ca(2+)-ATPase is essential
      for cell viability and tissue morphogenesis during development. Cultured cells
      treated with Ca(2+)-ATPase inhibitors exhibit impaired Notch cleavage and
      receptor trafficking to the cell surface, explaining the genetic interaction
      between Ca(2+)-ATPase and Notch. Notch and several other transmembrane proteins
      are mislocalized in tissue clones homozygous for Ca-P60A mutations, demonstrating
      a general effect on membrane protein trafficking caused by a deficiency in
      Ca(2+)-ATPase.
FAU - Periz, G
AU  - Periz G
AD  - Department of Genetics, Stellar-Chance Laboratories 709C, University of
      Pennsylvania School of Medicine, 422 Curie Boulevard, Philadelphia, PA 19104,
      USA.
FAU - Fortini, M E
AU  - Fortini ME
LA  - eng
GR  - AG14583/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Drosophila Proteins)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Indoles)
RN  - 0 (Membrane Proteins)
RN  - 0 (N protein, Drosophila)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Notch)
RN  - 67526-95-8 (Thapsigargin)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - X9TLY4580Z (cyclopiazonic acid)
SB  - IM
MH  - Animals
MH  - Calcium-Transporting ATPases/*metabolism
MH  - Cell Line
MH  - Darier Disease/genetics
MH  - Drosophila/*enzymology/genetics
MH  - Drosophila Proteins
MH  - Enzyme Inhibitors/pharmacology
MH  - Genes, Insect
MH  - Genes, Lethal
MH  - Humans
MH  - Immunohistochemistry
MH  - Indoles/pharmacology
MH  - Membrane Proteins/*metabolism
MH  - Microscopy, Electron, Scanning
MH  - Mutation
MH  - Phenotype
MH  - Receptors, Cell Surface/metabolism
MH  - Receptors, Notch
MH  - Signal Transduction
MH  - Thapsigargin/pharmacology
MH  - Transfection
PMC - PMC1171664
EDAT- 1999/11/02 00:00
MHDA- 1999/11/02 00:01
CRDT- 1999/11/02 00:00
PHST- 1999/11/02 00:00 [pubmed]
PHST- 1999/11/02 00:01 [medline]
PHST- 1999/11/02 00:00 [entrez]
AID - 10.1093/emboj/18.21.5983 [doi]
PST - ppublish
SO  - EMBO J. 1999 Nov 1;18(21):5983-93. doi: 10.1093/emboj/18.21.5983.