PMID- 10544261
OWN - NLM
STAT- MEDLINE
DCOM- 19991210
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 460
IP  - 2
DP  - 1999 Oct 29
TI  - Expression of the AMP-activated protein kinase beta1 and beta2 subunits in
      skeletal muscle.
PG  - 343-8
AB  - A heterotrimeric member of the AMP-activated protein kinase (AMPK) isoenzyme
      family was purified from rat skeletal muscle by immunoaffinity chromatography,
      consisting of an alpha2 catalytic and two non-catalytic subunits, beta2 and
      gamma1. The AMPK beta2 cDNA (271 amino acids (aa), molecular weight (MW)=30
      inverted question mark omitted inverted question mark307, pI 6. 3) was cloned
      from skeletal muscle and found to share an overall identity of 70% with beta1
      (270 aa, MW=30 inverted question mark omitted inverted question mark475, pI 6.0).
      In the liver AMPK beta1 subunit, Ser-182 is constitutively phosphorylated whereas
      in skeletal muscle beta2 isoform, we find that Ser-182 is only partially
      phosphorylated. In addition, the autophosphorylation sites Ser-24, Ser-25 found
      in the beta1 are replaced by Ala-Glu in the beta2 isoform. beta2 contains seven
      more Ser and one less Thr residues than beta1, raising the possibility of
      differential post-translational regulation. Immunoblot analysis further revealed 
      that soleus muscle (slow twitch) contains exclusively beta1 associated with
      alpha2, whereas extensor digitorum longus muscle alpha2 (EDL, fast twitch)
      associates with beta2 as well as beta1. Sequence analysis revealed that glycogen 
      synthase, a known AMPK substrate, co-immunoprecipitated with the AMPK
      alpha2beta2gamma1 complex.
FAU - Chen, Z
AU  - Chen Z
AD  - St. Vincent's Institute of Medical Research, St. Vincent's Hospital, 41 Victoria 
      Parade, Fitzroy, Vic., Australia.
FAU - Heierhorst, J
AU  - Heierhorst J
FAU - Mann, R J
AU  - Mann RJ
FAU - Mitchelhill, K I
AU  - Mitchelhill KI
FAU - Michell, B J
AU  - Michell BJ
FAU - Witters, L A
AU  - Witters LA
FAU - Lynch, G S
AU  - Lynch GS
FAU - Kemp, B E
AU  - Kemp BE
FAU - Stapleton, D
AU  - Stapleton D
LA  - eng
SI  - GENBANK/AF182717
GR  - DK35712/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA, Complementary)
RN  - 0 (Isoenzymes)
RN  - 0 (Multienzyme Complexes)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinases
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary/metabolism
MH  - Immunoblotting
MH  - Isoenzymes
MH  - Liver/enzymology
MH  - Male
MH  - Molecular Sequence Data
MH  - Multienzyme Complexes
MH  - Muscle, Skeletal/*enzymology
MH  - Protein Kinases/genetics/*metabolism
MH  - Protein-Serine-Threonine Kinases
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sequence Homology, Amino Acid
EDAT- 1999/11/02 00:00
MHDA- 1999/11/02 00:01
CRDT- 1999/11/02 00:00
PHST- 1999/11/02 00:00 [pubmed]
PHST- 1999/11/02 00:01 [medline]
PHST- 1999/11/02 00:00 [entrez]
AID - S001457939901371X [pii]
AID - 10.1016/s0014-5793(99)01371-x [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Oct 29;460(2):343-8. doi: 10.1016/s0014-5793(99)01371-x.