PMID- 10544250
OWN - NLM
STAT- MEDLINE
DCOM- 19991210
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 460
IP  - 2
DP  - 1999 Oct 29
TI  - Tropoelastin binding to fibulins, nidogen-2 and other extracellular matrix
      proteins.
PG  - 280-4
AB  - Elastic fibers in vessel walls and other tissues consist of cross-linked
      tropoelastin in association with several microfibrillar proteins. In order to
      understand the molecular basis of these structures, we examined the binding of
      recombinant human tropoelastin to other extracellular matrix ligands in solid
      phase binding and surface plasmon resonance assays. These studies demonstrated a 
      particularly high affinity (K(d) about 1 nM) of tropoelastin for microfibrillar
      fibulin-2 and the recently described nidogen-2 isoform. More moderate affinities 
      were observed for fibulin-1, laminin-1 and perlecan, while several other ligands 
      such as collagens, nidogen-1, fibronectin and BM-40 showed little or no binding. 
      In immunogold staining of mouse aortic media, elastic fibers were heavily
      decorated with tropoelastin, fibulin-2 and nidogen-2, while the reaction with
      fibulin-1 was lower. The colocalization of these proteins emphasizes the
      potential for in vivo interactions.
FAU - Sasaki, T
AU  - Sasaki T
AD  - Max-Planck-Institut fur Biochemie, Am Klopferspitz 18A, D-82152, Martinsried,
      Germany.
FAU - Gohring, W
AU  - Gohring W
FAU - Miosge, N
AU  - Miosge N
FAU - Abrams, W R
AU  - Abrams WR
FAU - Rosenbloom, J
AU  - Rosenbloom J
FAU - Timpl, R
AU  - Timpl R
LA  - eng
GR  - HL 56401/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Heparan Sulfate Proteoglycans)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (NID2 protein, human)
RN  - 0 (Proteoglycans)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Serum Albumin)
RN  - 0 (Tropoelastin)
RN  - 0 (fibulin)
RN  - 143972-95-6 (perlecan)
RN  - 9050-30-0 (Heparitin Sulfate)
SB  - IM
MH  - Animals
MH  - Aorta/metabolism
MH  - Calcium-Binding Proteins/*metabolism
MH  - Carrier Proteins/*metabolism
MH  - Cell Adhesion Molecules
MH  - Dose-Response Relationship, Drug
MH  - Extracellular Matrix Proteins/*metabolism
MH  - *Heparan Sulfate Proteoglycans
MH  - Heparitin Sulfate/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Membrane Glycoproteins/*metabolism
MH  - Mice
MH  - Protein Binding
MH  - Proteoglycans/metabolism
MH  - Recombinant Proteins/metabolism
MH  - Serum Albumin/metabolism
MH  - Surface Plasmon Resonance
MH  - Time Factors
MH  - Tropoelastin/*metabolism
EDAT- 1999/11/02 00:00
MHDA- 1999/11/02 00:01
CRDT- 1999/11/02 00:00
PHST- 1999/11/02 00:00 [pubmed]
PHST- 1999/11/02 00:01 [medline]
PHST- 1999/11/02 00:00 [entrez]
AID - S0014-5793(99)01362-9 [pii]
AID - 10.1016/s0014-5793(99)01362-9 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Oct 29;460(2):280-4. doi: 10.1016/s0014-5793(99)01362-9.