PMID- 10544099
OWN - NLM
STAT- MEDLINE
DCOM- 19991126
LR  - 20131121
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 263
IP  - 1
DP  - 1999 Oct 10
TI  - Chlorella virus PBCV-1 encodes a functional homospermidine synthase.
PG  - 254-62
AB  - Sequence analysis of the 330-kb genome of chlorella virus Paramecium bursaria
      chlorella virus 1 (PBCV-1) revealed an open reading frame, A237R, that encodes a 
      protein with 34% amino acid identity to homospermidine synthase from
      Rhodopseudomonas viridis. Expression of the a237r gene product in Escherichia
      coli established that the recombinant enzyme catalyzes the NAD(+)-dependent
      formation of homospermidine from two molecules of putrescine. The a237r gene is
      expressed late in PBCV-1 infection. Both uninfected and PBCV-1-infected
      chlorella, as well as PBCV-1 virions, contain homospermidine, along with the more
      common polyamines putrescine, spermidine, and cadaverine. The total number of
      polyamine molecules per virion ( approximately 539) is too small to significantly
      neutralize the virus double-stranded DNA (>660,000 nucleotides). Consequently,
      the biological significance of the homospermidine synthase gene is unknown.
      However, the gene is widespread among the chlorella viruses. To our knowledge,
      this is the first report of a virus encoding an enzyme involved in polyamine
      biosynthesis.
CI  - Copyright 1999 Academic Press.
FAU - Kaiser, A
AU  - Kaiser A
AD  - Institut fur Pharmazeutische Biologie, Technische Universitaet Braunschweig,
      Mendelssohnstrasse 1, Braunschweig, Germany.
FAU - Vollmert, M
AU  - Vollmert M
FAU - Tholl, D
AU  - Tholl D
FAU - Graves, M V
AU  - Graves MV
FAU - Gurnon, J R
AU  - Gurnon JR
FAU - Xing, W
AU  - Xing W
FAU - Lisec, A D
AU  - Lisec AD
FAU - Nickerson, K W
AU  - Nickerson KW
FAU - Van Etten, J L
AU  - Van Etten JL
LA  - eng
SI  - GENBANK/U42580
GR  - GM-32441/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (DNA, Viral)
RN  - 0 (Polyamines)
RN  - 0 (Recombinant Proteins)
RN  - 9007-49-2 (DNA)
RN  - EC 2.5.- (Alkyl and Aryl Transferases)
RN  - EC 2.5.1.- (homospermidine synthetase)
RN  - EC 4.1.1.17 (Ornithine Decarboxylase)
RN  - U87FK77H25 (Spermidine)
SB  - IM
MH  - Alkyl and Aryl Transferases/chemistry/*genetics/metabolism
MH  - Amino Acid Sequence
MH  - Chlorella/*virology
MH  - DNA/analysis
MH  - DNA, Viral/analysis/genetics
MH  - Genes, Viral
MH  - Molecular Sequence Data
MH  - Ornithine Decarboxylase/metabolism
MH  - Phycodnaviridae/*enzymology/*genetics
MH  - Plant Diseases/*virology
MH  - Polyamines/metabolism
MH  - Recombinant Proteins/metabolism
MH  - Spermidine/biosynthesis
MH  - Virion
EDAT- 1999/11/02 00:00
MHDA- 1999/11/02 00:01
CRDT- 1999/11/02 00:00
PHST- 1999/11/02 00:00 [pubmed]
PHST- 1999/11/02 00:01 [medline]
PHST- 1999/11/02 00:00 [entrez]
AID - 10.1006/viro.1999.9972 [doi]
AID - S0042-6822(99)99972-5 [pii]
PST - ppublish
SO  - Virology. 1999 Oct 10;263(1):254-62. doi: 10.1006/viro.1999.9972.