PMID- 10544043
OWN - NLM
STAT- MEDLINE
DCOM- 19991222
LR  - 20081121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 264
IP  - 3
DP  - 1999 Nov 2
TI  - Cloning and functional characterization of the promoter region of the gene
      encoding human adenylate kinase isozyme 3.
PG  - 990-7
AB  - The 5'-flanking region of the gene encoding human adenylate kinase isozyme 3 was 
      isolated and compared with that of the bovine AK3 gene previously characterized. 
      Four conserved DNA sequences (elements-a, -b, -c, and -d) were found in both the 
      regions. The promoter activities were analyzed in HeLa cells using promoter-CAT
      reporter constructs. The proximal promoter region (-217 to +261), which contains 
      three of four conserved elements, gave a maximum promoter activity. In a series
      of electrophoretic mobility-shift assays, DNA fragments and double-stranded
      oligodeoxyribonucleotides containing sequences of the four conserved elements
      interacted with nuclear extracts of HeLa cells. The a-element contained the
      W-element, while the d-element, which had a high G + C content, was a novel
      regulatory cis-element distinct from the GC box. The b- and c-elements were
      homologous to each other and had a motif resembling downstream promoter element. 
      Mutations of the c- and d-elements significantly reduced the promoter activity,
      indicating that the c- and d-elements in the AK3 promoter are crucial. These
      elements may also be involved in the transcriptional regulation of other
      TATA-less genes.
CI  - Copyright 1999 Academic Press.
FAU - Noma, T
AU  - Noma T
AD  - Department of Biochemistry, Yamaguchi University School of Medicine, 1-1-1
      Minamikogushi, Ube, Yamaguchi, 755-8505, Japan. tnoma@po.cc.yamaguchi-u.ac.jp
FAU - Adachi, N
AU  - Adachi N
FAU - Nakazawa, A
AU  - Nakazawa A
LA  - eng
SI  - GENBANK/D37992
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Isoenzymes)
RN  - EC 2.7.4.3 (Adenylate Kinase)
SB  - IM
MH  - Adenylate Kinase/*genetics
MH  - Animals
MH  - Base Sequence
MH  - Cattle
MH  - Cloning, Molecular
MH  - Gene Expression Regulation, Enzymologic
MH  - Humans
MH  - Isoenzymes/genetics
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic/*genetics
EDAT- 1999/11/02 00:00
MHDA- 1999/11/02 00:01
CRDT- 1999/11/02 00:00
PHST- 1999/11/02 00:00 [pubmed]
PHST- 1999/11/02 00:01 [medline]
PHST- 1999/11/02 00:00 [entrez]
AID - 10.1006/bbrc.1999.1616 [doi]
AID - S0006-291X(99)91616-9 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Nov 2;264(3):990-7. doi: 10.1006/bbrc.1999.1616.