PMID- 10544017
OWN - NLM
STAT- MEDLINE
DCOM- 19991222
LR  - 20041117
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 264
IP  - 3
DP  - 1999 Nov 2
TI  - Identification of three new alternate human kallikrein 2 transcripts: evidence of
      long transcript and alternative splicing.
PG  - 833-9
AB  - In a search for prostate-specific genes in the human expressed sequence tag (EST)
      database, we identified a seemingly unique EST cluster C81. Experimental data
      linked C81 to the human hKLK2 gene that encodes a prostate specific serine
      protease-human glandular kallikrein (hK2). We uncovered a full-length hKLK2 cDNA 
      corresponding to a 3.0 kb hKLK2 mRNA by PCR and sequence analysis. The 3.0 kb
      transcript accounts for about 25% of the hKLK2 transcripts as compared to the
      previously known 1.5 kb transcript. We also identified a third spliced form of
      the hKLK2 gene produced by alternative splicing between intron III and exon 4.
      This spliced form was detected in normal prostate, prostate cancer and the
      prostate adenocarcinoma cell line LNCaP. The identification of long hKLK2
      transcript and an alternative spliced form of the hKLK2 gene indicates that
      regulation of the gene is complex.
CI  - Copyright 1999 Academic Press.
FAU - Liu, X F
AU  - Liu XF
AD  - Laboratory of Molecular Biology, Division of Basic Sciences, National Institutes 
      of Health, Building 37, Room 4E16, Bethesda, Maryland, 20892, USA.
FAU - Essand, M
AU  - Essand M
FAU - Vasmatzis, G
AU  - Vasmatzis G
FAU - Lee, B
AU  - Lee B
FAU - Pastan, I
AU  - Pastan I
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA, Complementary)
RN  - EC 3.4.21.35 (Tissue Kallikreins)
SB  - IM
MH  - *Alternative Splicing
MH  - Base Sequence
MH  - DNA, Complementary/genetics/isolation & purification
MH  - Humans
MH  - Molecular Sequence Data
MH  - Tissue Kallikreins/*genetics
MH  - Transcription, Genetic
EDAT- 1999/11/02 00:00
MHDA- 1999/11/02 00:01
CRDT- 1999/11/02 00:00
PHST- 1999/11/02 00:00 [pubmed]
PHST- 1999/11/02 00:01 [medline]
PHST- 1999/11/02 00:00 [entrez]
AID - 10.1006/bbrc.1999.1595 [doi]
AID - S0006-291X(99)91595-4 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Nov 2;264(3):833-9. doi: 10.1006/bbrc.1999.1595.