PMID- 10542369
OWN - NLM
STAT- MEDLINE
DCOM- 20000107
LR  - 20200319
IS  - 0886-1544 (Print)
IS  - 0886-1544 (Linking)
VI  - 44
IP  - 3
DP  - 1999 Nov
TI  - Phosphorylation of MAP2c and MAP4 by MARK kinases leads to the destabilization of
      microtubules in cells.
PG  - 209-24
AB  - Microtubules serve as transport tracks in molecular mechanisms governing cellular
      shape and polarity. Rapid transitions between stable and dynamic microtubules are
      regulated by several factors, including microtubule-associated proteins (MAPs).
      We have shown that MAP/microtubule affinity regulating kinases (MARK) can
      phosphorylate the microtubule-associated-proteins MAP4, MAP2c, and tau on their
      microtubule-binding domain in vitro. This leads to their detachment from
      microtubules (MT) and an increased dynamic instability of MT. Here we show that
      MARK protein kinases phosphorylate MAP2 and MAP4 on their microtubule-binding
      domain in transfected CHO cells. In CHO cells expressing MARK1 or MARK2 under
      control of an inducible promoter, MARK2 phosphorylates an endogenous MAP4-related
      protein. Prolonged expression of MARK2 results in microtubule-disruption,
      detachment of cells from the substratum, and cell death. Concomitant with
      microtubule disruption, we also observed a breakdown of the vimentin network,
      whereas actin fibers remained unaffected. Thus, MARK seems to play an important
      role in controlling cytoskeletal dynamics.
CI  - Copyright 1999 Wiley-Liss, Inc.
FAU - Ebneth, A
AU  - Ebneth A
AD  - Max-Planck Unit for Structural Molecular Biology, Hamburg, Germany.
FAU - Drewes, G
AU  - Drewes G
FAU - Mandelkow, E M
AU  - Mandelkow EM
FAU - Mandelkow, E
AU  - Mandelkow E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Motil Cytoskeleton
JT  - Cell motility and the cytoskeleton
JID - 8605339
RN  - 0 (Actins)
RN  - 0 (Epitopes, B-Lymphocyte)
RN  - 0 (MAP2 protein, human)
RN  - 0 (MAP2 protein, rat)
RN  - 0 (MAP4)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Mtap2 protein, mouse)
RN  - 0 (tau Proteins)
RN  - EC 2.7.1.- (MARK2 protein, rat)
RN  - EC 2.7.1.- (Mark1 protein, rat)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
SB  - IM
MH  - Actins/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - CHO Cells
MH  - Cell Division
MH  - Cricetinae
MH  - Epitopes, B-Lymphocyte/immunology
MH  - Intermediate Filaments/metabolism
MH  - Mice
MH  - Microtubule-Associated Proteins/genetics/*metabolism
MH  - Microtubules/*metabolism/ultrastructure
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - Protein-Serine-Threonine Kinases/genetics/*metabolism
MH  - tau Proteins/metabolism
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
AID - 10.1002/(SICI)1097-0169(199911)44:3<209::AID-CM6>3.0.CO;2-4 [pii]
AID - 10.1002/(SICI)1097-0169(199911)44:3<209::AID-CM6>3.0.CO;2-4 [doi]
PST - ppublish
SO  - Cell Motil Cytoskeleton. 1999 Nov;44(3):209-24. doi:
      10.1002/(SICI)1097-0169(199911)44:3<209::AID-CM6>3.0.CO;2-4.