PMID- 10542210
OWN - NLM
STAT- MEDLINE
DCOM- 19991213
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 45
DP  - 1999 Nov 5
TI  - Expression of the gene for mitoribosomal protein S12 is controlled in human cells
      at the levels of transcription, RNA splicing, and translation.
PG  - 31853-62
AB  - The human gene RPMS12 encodes a protein similar to bacterial ribosomal protein
      S12 and is proposed to represent the human mitochondrial orthologue. RPMS12
      reporter gene expression in cultured human cells supports the idea that the gene 
      product is mitochondrial and is localized to the inner membrane. Human cells
      contain at least four structurally distinct RPMS12 mRNAs that differ in their
      5'-untranslated region (5'-UTR) as a result of alternate splicing and of 5' end
      heterogeneity. All of them encode the same polypeptide. The full 5'-UTR contains 
      two types of sequence element implicated elsewhere in translational regulation as
      follows: a short upstream open reading frame and an oligopyrimidine tract similar
      to that found at the 5' end of mRNAs encoding other growth-regulated proteins,
      including those of cytosolic ribosomes. The fully spliced (short) mRNA is the
      predominant form in all cell types studied and is translationally down-regulated 
      in cultured cells in response to serum starvation, even though it lacks both of
      the putative translational regulatory elements. By contrast, other splice
      variants containing one or both of these elements are not translationally
      regulated by growth status but are translated poorly in both growing and
      non-growing cells. Reporter analysis identified a 26-nucleotide tract of the
      5'-UTR of the short mRNA that is essential for translational down-regulation in
      growth-inhibited cells. Such experiments also confirmed that the 5'-UTR of the
      longer mRNA variants contains negative regulatory elements for translation.
      Tissue representation of RPMS12 mRNA is highly variable, following a typical
      mitochondrial pattern, but the relative levels of the different splice variants
      are similar in different tissues. These findings indicate a complex, multilevel
      regulation of RPMS12 gene expression in response to signals mediating growth,
      tissue specialization, and probably metabolic needs.
FAU - Mariottini, P
AU  - Mariottini P
AD  - Department of Biology, Universita di "Roma Tre," Rome, I-00146, Italy.
FAU - Shah, Z H
AU  - Shah ZH
FAU - Toivonen, J M
AU  - Toivonen JM
FAU - Bagni, C
AU  - Bagni C
FAU - Spelbrink, J N
AU  - Spelbrink JN
FAU - Amaldi, F
AU  - Amaldi F
FAU - Jacobs, H T
AU  - Jacobs HT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Ribosomal Proteins)
RN  - 0 (ribosomal protein S12)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cells, Cultured
MH  - *Gene Expression Regulation
MH  - HeLa Cells
MH  - Humans
MH  - Mitochondria/metabolism
MH  - Molecular Sequence Data
MH  - *Protein Biosynthesis
MH  - *RNA Splicing
MH  - Ribosomal Proteins/*genetics
MH  - *Transcription, Genetic
MH  - Xenopus
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
AID - 10.1074/jbc.274.45.31853 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Nov 5;274(45):31853-62. doi: 10.1074/jbc.274.45.31853.