PMID- 10542198
OWN - NLM
STAT- MEDLINE
DCOM- 19991213
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 45
DP  - 1999 Nov 5
TI  - Rapid STAT phosphorylation via the B cell receptor. Modulatory role of CD19.
PG  - 31770-4
AB  - Engagement of the B cell receptor (BCR) initiates multiple signaling cascades
      which mediate different biological responses, depending on the stage of B cell
      differentiation, antigen binding affinity, and duration of stimulation.
      Aggregation of co-receptors such as CD19 with the antigen receptor has been
      suggested to modulate the signals necessary for the development and functioning
      of the humoral immune system. In this study, we demonstrate that engagement of
      the antigen receptor on peripheral blood B cells, but not naive splenic B
      lymphocytes, leads to rapid phosphorylation of signal transducers and activators 
      of transcription 1 (STAT1) on Tyr-701 and Ser-727. Interestingly, phosphorylation
      on tyrosine diminished with increased stimulation, whereas serine phosphorylation
      correlated directly with the level of BCR cross-linking. In contrast,
      phosphorylation of STAT3 occurs exclusively on serine and is sensitive to
      inhibitors of the PI3-kinase and the ERK1/2 pathways. Finally, we show that
      co-ligation of CD19 with the BCR results in increased tyrosine phosphorylation of
      STAT1 relative to BCR cross-linking alone, establishing CD19 as a positive
      modulator of BCR-mediated STAT activation.
FAU - Su, L
AU  - Su L
AD  - Department of Biology, University of California San Diego, La Jolla, California
      92093-0322, USA.
FAU - Rickert, R C
AU  - Rickert RC
FAU - David, M
AU  - David M
LA  - eng
GR  - R01 CA080105/CA/NCI NIH HHS/United States
GR  - R01 CA080105-01/CA/NCI NIH HHS/United States
GR  - R01 CA080105-02/CA/NCI NIH HHS/United States
GR  - R01 CA080105-03/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Acute-Phase Proteins)
RN  - 0 (Antigens, CD19)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Receptors, Antigen, B-Cell)
RN  - 0 (STAT1 Transcription Factor)
RN  - 0 (STAT1 protein, human)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (STAT3 protein, human)
RN  - 0 (Trans-Activators)
RN  - 42HK56048U (Tyrosine)
RN  - 452VLY9402 (Serine)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.25 (MAP Kinase Kinase Kinase 1)
RN  - EC 2.7.11.25 (MAP Kinase Kinase Kinases)
RN  - EC 2.7.11.25 (MAP3K1 protein, human)
SB  - IM
MH  - Acute-Phase Proteins/*metabolism
MH  - Antigens, CD19/*metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - Humans
MH  - *MAP Kinase Kinase Kinase 1
MH  - MAP Kinase Kinase Kinases/metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphorylation
MH  - *Protein-Serine-Threonine Kinases
MH  - Receptors, Antigen, B-Cell/*metabolism
MH  - STAT1 Transcription Factor
MH  - STAT3 Transcription Factor
MH  - Serine/metabolism
MH  - *Signal Transduction
MH  - Trans-Activators/*metabolism
MH  - Tyrosine/metabolism
PMC - PMC2772110
MID - NIHMS145350
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
AID - 10.1074/jbc.274.45.31770 [doi]
AID - S0021-9258(19)51486-7 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Nov 5;274(45):31770-4. doi: 10.1074/jbc.274.45.31770.