PMID- 10542075
OWN - NLM
STAT- MEDLINE
DCOM- 20000110
LR  - 20191210
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 266
IP  - 1
DP  - 1999 Nov
TI  - Functional analysis of the 5'-flanking region of FTA for expression of rat
      GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase.
PG  - 274-81
AB  - The tissue-specific and species-specific expression of the ABH antigens is well
      known among vertebrate species and it is regulated by the
      alpha(1,2)fucosyltransferase that forms the H antigen, a precursor of the A and B
      antigens. To investigate the mechanisms governing the tissue-specific and
      species-specific expression of this alpha(1,2)fucosyltransferase, we
      characterized the gene structure, including the promoter region, of FTA, a rat
      orthologous homolog of human FUT1 that encodes the H alpha(1,
      2)fucosyltransferase and is responsible for the expression of the ABH antigens on
      human red blood cells. Northern blot and 5'-RACE analyses suggested that at least
      two forms of FTA mRNA (2.9 and 2.6 kb), which use alternative transcription start
      sites, are present in the cancer cell lines RCN-9 (rat colon cancer) and PC12
      (rat pheochromocytoma), whereas only the 2.6 kb form was detected in normal
      colon, stomach and pancreas. Transcriptional activity of the 5'-flanking
      sequence, which contains three putative Sp1-binding sites, but lacks both TATA
      and CAAT boxes, was examined. Transient transfection experiments of
      promoter-reporter gene constructs showed high promoter activity in RCN-9, PC12
      and human colon cancer (WiDr) cell lines, weak activity in human vascular
      endothelial (ECV304) cells and no activity in human erythroleukemia (HEL) cells. 
      The results suggest that the 5'-flanking region of FTA contains a tissue-specific
      promoter. Deletional analysis of the 5'-flanking sequence revealed regions
      containing cell-type-specific positive acting element(s) and negative regulatory 
      element(s), which are related to the promoter activity.
FAU - Soejima, M
AU  - Soejima M
AD  - Division of Human Genetics, Department of Forensic Medicine, Kurume University
      School of Medicine, Kurume, Japan.
FAU - Koda, Y
AU  - Koda Y
FAU - Wang, B
AU  - Wang B
FAU - Kimura, H
AU  - Kimura H
LA  - eng
SI  - GENBANK/AB006137
SI  - GENBANK/AB015637
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 2.4.1.- (Fucosyltransferases)
RN  - EC 2.4.1.69 (galactoside 2-alpha-L-fucosyltransferase)
SB  - IM
MH  - *5' Untranslated Regions
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - COS Cells
MH  - Chlorocebus aethiops
MH  - Endothelium, Vascular/cytology/metabolism
MH  - Enzyme Induction
MH  - Fucosyltransferases/biosynthesis/genetics
MH  - Genes
MH  - HeLa Cells
MH  - Humans
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/biosynthesis/genetics
MH  - Organ Specificity
MH  - PC12 Cells
MH  - Polymerase Chain Reaction
MH  - Promoter Regions, Genetic
MH  - Rats
MH  - Recombinant Fusion Proteins/biosynthesis
MH  - Regulatory Sequences, Nucleic Acid
MH  - Sequence Deletion
MH  - Species Specificity
MH  - Transfection
EDAT- 1999/10/29 00:00
MHDA- 1999/10/29 00:01
CRDT- 1999/10/29 00:00
PHST- 1999/10/29 00:00 [pubmed]
PHST- 1999/10/29 00:01 [medline]
PHST- 1999/10/29 00:00 [entrez]
AID - ejb865 [pii]
AID - 10.1046/j.1432-1327.1999.00865.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Nov;266(1):274-81. doi: 10.1046/j.1432-1327.1999.00865.x.