PMID- 10542057
OWN - NLM
STAT- MEDLINE
DCOM- 20000110
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 266
IP  - 1
DP  - 1999 Nov
TI  - Structural studies on lipopolysaccharides of serologically non-typable strains of
      Helicobacter pylori, AF1 and 007, expressing Lewis antigenic determinants.
PG  - 123-31
AB  - In contrast to other Helicobacter pylori strains, which have serologically
      detectable Lewis(x)+ (Le(x)) and Lewis(y)++ (++Le(y)) antigenic determinants in
      the O-specific polysaccharide chains of the lipopolysaccharides, H. pylori AF1
      and 007 were non-typable with anti-Le(x) and anti-Le(y) antibodies. The
      carbohydrate portions of the lipopolysaccharides were liberated by mild acid
      hydrolysis and subsequently studied by sugar and methylation analyses, 1H-NMR
      spectroscopy and electrospray ionization-mass spectrometry. Compared with each
      other, and with lipopolysaccharides of strains studied previously, the
      lipopolysaccharides of both AF1 and 007 showed similarities, but also
      differences, in the structures of the core region and O-specific polysaccharide
      chains. The O-specific polysaccharide chains of both strains consisted of a short
      or long polyfucosylated poly-N-acetyl-beta-lactosamine chains, which were
      distinguished from those of other strains by a high degree of fucosylation
      producing a polymeric Le(x)chain terminating with Le(x) or Le(y) units:[sequence:
      see text] where n = 0 or 1 in strain AF1 and 0 in strain 007, m = 0-2, 6-7 in
      strain AF1 and m = 0-2, 6-7 or approximately 40 in strain 007, the medium-size
      species being predominant. Therefore, compared with other strains, the lack of
      reactivity of lipopolysaccharide of H. pylori AF1 and 007 with anti-Le(x) and
      anti-Le(y) may reflect the presence of a polymeric Le(x) chain and has important 
      implications for serological and pathogenesis studies. As the substitution
      pattern of a D-glycero-D-manno-heptose residue in the outer core varied in the
      two strains, and an extended DD-heptan chain was present in some
      lipopolysaccharide species but not in others, this region was less conservative
      than the inner core region. The inner core L-glycero-D-manno-heptose region of
      both strains carried a 2-aminoethyl phosphate group, rather than a phosphate
      group, as reported previously for other H. pylori strains.
FAU - Knirel, Y A
AU  - Knirel YA
AD  - Karolinska Institute, Clinical Research Center, Huddinge University Hospital,
      Sweden.
FAU - Kocharova, N A
AU  - Kocharova NA
FAU - Hynes, S O
AU  - Hynes SO
FAU - Widmalm, G
AU  - Widmalm G
FAU - Andersen, L P
AU  - Andersen LP
FAU - Jansson, P E
AU  - Jansson PE
FAU - Moran, A P
AU  - Moran AP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Carbohydrates)
RN  - 0 (Lewis Blood-Group System)
RN  - 0 (Lewis X Antigen)
RN  - 0 (Lewis Y antigen)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Oligosaccharides)
SB  - IM
MH  - Antigens, Bacterial/*chemistry/immunology
MH  - Carbohydrate Conformation
MH  - Carbohydrate Sequence
MH  - Carbohydrates/chemistry
MH  - Helicobacter pylori/*chemistry/classification/immunology
MH  - Lewis Blood-Group System/*analysis
MH  - Lewis X Antigen/*analysis
MH  - Lipopolysaccharides/*chemistry/immunology
MH  - Magnetic Resonance Spectroscopy
MH  - Mass Spectrometry
MH  - Molecular Sequence Data
MH  - Oligosaccharides/*chemistry/isolation & purification
MH  - Serotyping
EDAT- 1999/10/29 00:00
MHDA- 1999/10/29 00:01
CRDT- 1999/10/29 00:00
PHST- 1999/10/29 00:00 [pubmed]
PHST- 1999/10/29 00:01 [medline]
PHST- 1999/10/29 00:00 [entrez]
AID - ejb831 [pii]
AID - 10.1046/j.1432-1327.1999.00831.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Nov;266(1):123-31. doi: 10.1046/j.1432-1327.1999.00831.x.