PMID- 10540346
OWN - NLM
STAT- MEDLINE
DCOM- 19991116
LR  - 20180423
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 29
IP  - 10
DP  - 1999 Oct
TI  - Expansion of circulating V gamma 9/V delta 1 T cells in a patient with a syndrome
      of recurrent fever: evidence for an unusual antigen-driven process leading to
      selection of recurrent motifs within TCR junctional loops of diverse lengths.
PG  - 3338-49
AB  - Polyclonal expansions of human Vdelta1 T cells have been described in diverse
      physiopathological situations without strong TCR structural data for an
      antigen-driven selection. Here, we have analyzed the phenotype and TCR repertoire
      of gamma delta T cells obtained from the peripheral blood of a 19-year-old
      patient with a syndrome of recurrent fever, which accounted for up to 40% of
      CD3(+) T cells and expressed predominantly Vgamma9 and Vdelta1 TCR regions and a 
      memory phenotype. Sequence analysis of Vdelta1-Jdelta1 transcripts derived from
      peripheral blood lymphocytes (PBL) indicated that, while Vdelta1-Jdelta1
      junctional sequences were diverse in length, all but one contained several
      recurrent motifs at conserved positions from both the 5'- and 3'-ends of the
      complementarity-determining region (CDR)3 loop. Analysis of gamma delta T cell
      clones derived from patient PBL demonstrated that Vgamma9(+) but not Vgamma9(-) T
      cell clones frequently expressed Vdelta1 chains with these characteristics and
      unveiled a hierarchy between the constraints imposed on the 5'- vs. the 3' motifs
      of the Vdelta1 CDR3 loops. These results constitute the first strong evidence for
      a nominal antigen-driven selection of Vdelta1 T cells in vivo and also suggest
      that the hierarchy of the constraints imposed by antigens respectively on the
      length and amino acid composition of TCR CDR3 loops differs between alpha beta
      and gamma delta T cells.
FAU - Jouen-Beades, F
AU  - Jouen-Beades F
AD  - INSERM U519 Institut Federatif de Recherche Multidisciplinaire sur les Peptides
      (IFR23), Faculte Mixte de Medecine et de Pharmacie, Hopital Charles Nicolle,
      Rouen, France.
FAU - Halary, F
AU  - Halary F
FAU - Drouot, L
AU  - Drouot L
FAU - Peyrat, M A
AU  - Peyrat MA
FAU - Paris, E
AU  - Paris E
FAU - Joly, P
AU  - Joly P
FAU - Gilbert, D
AU  - Gilbert D
FAU - Bonneville, M
AU  - Bonneville M
FAU - Tron, F
AU  - Tron F
LA  - eng
SI  - GENBANK/AJ132111
SI  - GENBANK/AJ132112
SI  - GENBANK/AJ132113
SI  - GENBANK/AJ132114
SI  - GENBANK/AJ132115
SI  - GENBANK/AJ132116
SI  - GENBANK/AJ132117
SI  - GENBANK/AJ132118
SI  - GENBANK/AJ132119
SI  - GENBANK/AJ132120
SI  - GENBANK/AJ132121
SI  - GENBANK/AJ132122
SI  - GENBANK/AJ132123
SI  - GENBANK/AJ132124
SI  - GENBANK/AJ132125
SI  - GENBANK/AJ132126
SI  - GENBANK/AJ132127
SI  - GENBANK/AJ132128
SI  - GENBANK/AJ132129
SI  - GENBANK/AJ132783
SI  - GENBANK/AJ132784
SI  - GENBANK/AJ132785
SI  - GENBANK/AJ132786
SI  - GENBANK/AJ132787
SI  - GENBANK/AJ132788
SI  - GENBANK/AJ132789
SI  - GENBANK/AJ132790
SI  - GENBANK/AJ132831
SI  - GENBANK/AJ132832
SI  - GENBANK/AJ132833
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Receptors, Antigen, T-Cell, gamma-delta)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence/genetics
MH  - Clone Cells
MH  - Fever of Unknown Origin/blood/*immunology
MH  - Humans
MH  - Molecular Sequence Data
MH  - Protein Conformation
MH  - Receptors, Antigen, T-Cell, gamma-delta/*blood/genetics
MH  - *Repetitive Sequences, Amino Acid
MH  - *Repetitive Sequences, Nucleic Acid
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Syndrome
MH  - T-Lymphocyte Subsets/*metabolism
EDAT- 1999/10/30 00:00
MHDA- 1999/10/30 00:01
CRDT- 1999/10/30 00:00
PHST- 1999/10/30 00:00 [pubmed]
PHST- 1999/10/30 00:01 [medline]
PHST- 1999/10/30 00:00 [entrez]
AID - 10.1002/(SICI)1521-4141(199910)29:10<3338::AID-IMMU3338>3.0.CO;2-B [doi]
PST - ppublish
SO  - Eur J Immunol. 1999 Oct;29(10):3338-49. doi:
      10.1002/(SICI)1521-4141(199910)29:10<3338::AID-IMMU3338>3.0.CO;2-B.