PMID- 10540326
OWN - NLM
STAT- MEDLINE
DCOM- 19991116
LR  - 20180424
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 29
IP  - 10
DP  - 1999 Oct
TI  - Molecular and functional characterization of IRp60, a member of the
      immunoglobulin superfamily that functions as an inhibitory receptor in human NK
      cells.
PG  - 3148-59
AB  - In this study we describe the functional and molecular characterization of IRp60 
      (inhibitory receptor protein 60), an inhibitory receptor expressed on all human
      NK cells. The IRp60 molecule has been identified by the generation of three novel
      monoclonal antibodies (mAb). Cross-linking of IRp60 by specific mAb strongly
      inhibits the spontaneous cytotoxicity of NK cells as well as the NK-mediated
      cytolytic activity induced via different non-HLA-specific or HLA-specific
      activating receptors. IRp60 is a 60-kDa glycoprotein that, upon sodium
      pervanadate treatment, becomes tyrosine phosphorylated and associates with the
      SH2-containing phosphatases SHP-1 and SHP-2. The IRp60 gene is located on human
      chromosome 17 and encodes a molecule belonging to the immunoglobulin (Ig)
      superfamily characterized by a single V-type Ig-like domain in the extracellular 
      portion. The cytoplasmic tail contains three classical immunoreceptor
      tyrosine-based inhibitory motifs. Southern blot analysis revealed
      cross-hybridization with monkey and mouse genomic DNA, thus suggesting that IRp60
      may be conserved among different species. Moreover, based on the use of different
      anti-IRp60 mAb, we could identify two IRp60 allelic variants. Since IRp60 is also
      expressed by other cell types, including T cell subsets, monocytes and
      granulocytes, it may play a more general role in the negative regulation of
      different leukocyte populations.
FAU - Cantoni, C
AU  - Cantoni C
AD  - Dipartimento di Medicina Sperimentale, Universita degli Studi di Genova, Genova, 
      Italy.
FAU - Bottino, C
AU  - Bottino C
FAU - Augugliaro, R
AU  - Augugliaro R
FAU - Morelli, L
AU  - Morelli L
FAU - Marcenaro, E
AU  - Marcenaro E
FAU - Castriconi, R
AU  - Castriconi R
FAU - Vitale, M
AU  - Vitale M
FAU - Pende, D
AU  - Pende D
FAU - Sivori, S
AU  - Sivori S
FAU - Millo, R
AU  - Millo R
FAU - Biassoni, R
AU  - Biassoni R
FAU - Moretta, L
AU  - Moretta L
FAU - Moretta, A
AU  - Moretta A
LA  - eng
SI  - GENBANK/AJ224864
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Antigens, CD)
RN  - 0 (CD300A protein, human)
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Complementary)
RN  - 0 (Immunoglobulins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, KIR)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, CD
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 17
MH  - Cloning, Molecular
MH  - DNA Probes
MH  - DNA, Complementary/analysis
MH  - Haplorhini
MH  - Humans
MH  - Immunoglobulins/*chemistry/genetics
MH  - Killer Cells, Natural/*metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - RNA, Messenger/biosynthesis
MH  - Receptors, Immunologic/*chemistry/genetics/metabolism
MH  - Receptors, KIR
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
EDAT- 1999/10/30 00:00
MHDA- 1999/10/30 00:01
CRDT- 1999/10/30 00:00
PHST- 1999/10/30 00:00 [pubmed]
PHST- 1999/10/30 00:01 [medline]
PHST- 1999/10/30 00:00 [entrez]
AID - 10.1002/(SICI)1521-4141(199910)29:10<3148::AID-IMMU3148>3.0.CO;2-L [doi]
PST - ppublish
SO  - Eur J Immunol. 1999 Oct;29(10):3148-59. doi:
      10.1002/(SICI)1521-4141(199910)29:10<3148::AID-IMMU3148>3.0.CO;2-L.