PMID- 10540230
OWN - NLM
STAT- MEDLINE
DCOM- 19991130
LR  - 20190513
IS  - 0019-2805 (Print)
IS  - 0019-2805 (Linking)
VI  - 98
IP  - 2
DP  - 1999 Oct
TI  - Cell surface expression and metabolism of major histocompatibility complex class 
      II invariant chain (CD74) by diverse cell lines.
PG  - 296-302
AB  - We previously described the processing of antibodies to CD74 (the major
      histocompatibility complex class II-associated invariant chain, Ii), by B-cell
      lymphoma cell lines. These cells expressed relatively low levels of Ii on the
      surface, but the molecules were rapidly internalized and replaced by new
      molecules, so that approximately 8 x 10(6) antibody molecules per cell were taken
      up per day. We herein report the results of similar studies with other cell
      types, namely a melanoma, a colon carcinoma, a T-cell lymphoma and
      B-lymphoblastoid cell lines. The melanoma and the carcinoma were treated with
      interferon-gamma to induce high levels of the antigen. The T-cell lymphoma, HUT
      78, was selected specifically because it was previously reported to lack cell
      surface Ii, while expressing the molecule intracellularly. However, HUT 78
      displayed Ii on the cell surface, as did the other cell lines tested, and
      catabolism of the antibody was very fast on all of the cell lines. The capacity
      of four of the cell lines for cumulative antibody uptake was evaluated, using
      'residualizing' radiolabels, which are trapped within the cell after catabolism
      of the antibody to which they were conjugated. A high level of uptake was
      observed in all cases, although there was significant variation between the cell 
      lines. With melanoma SK-MEL-37, the total LL1 uptake in 24 hr was nearly 10(7)
      molecules per cell and the average turnover time for Ii on the cell surface was 4
      min; with carcinoma HT-29, the total LL1 uptake in 24 hr was approximately 10(6) 
      molecules per cell, and the average turnover time for Ii on the cell surface was 
      27 min. Based on the cell content of mature class II antigens (alphabeta), these 
      data suggest that a large fraction, or all, of immature class II molecules
      (alphabetaIi) reach the cell surface before entering the peptide-loading
      compartment, independent of the particular cell type.
FAU - Ong, G L
AU  - Ong GL
AD  - Garden State Cancer Center at the Center for Molecular Medicine and Immunology,
      Belleville, NJ 07109, USA.
FAU - Goldenberg, D M
AU  - Goldenberg DM
FAU - Hansen, H J
AU  - Hansen HJ
FAU - Mattes, M J
AU  - Mattes MJ
LA  - eng
GR  - CA39841/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, B-Lymphocyte)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (invariant chain)
SB  - IM
MH  - *Antigen-Antibody Reactions
MH  - Antigens, CD/*analysis/metabolism
MH  - Antigens, Differentiation, B-Lymphocyte/*analysis/metabolism
MH  - Colonic Neoplasms/immunology
MH  - Histocompatibility Antigens Class II/*analysis/metabolism
MH  - Humans
MH  - Lymphoma, B-Cell/immunology
MH  - Lymphoma, T-Cell/immunology
MH  - Melanoma/immunology
MH  - Tumor Cells, Cultured/*immunology
PMC - PMC2326920
EDAT- 1999/12/14 00:00
MHDA- 1999/12/14 00:01
CRDT- 1999/12/14 00:00
PHST- 1999/12/14 00:00 [pubmed]
PHST- 1999/12/14 00:01 [medline]
PHST- 1999/12/14 00:00 [entrez]
AID - imm868 [pii]
AID - 10.1046/j.1365-2567.1999.00868.x [doi]
PST - ppublish
SO  - Immunology. 1999 Oct;98(2):296-302. doi: 10.1046/j.1365-2567.1999.00868.x.