PMID- 10537111
OWN - NLM
STAT- MEDLINE
DCOM- 19991102
LR  - 20061115
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 401
IP  - 6754
DP  - 1999 Oct 14
TI  - Atomic structure of the GCSF-receptor complex showing a new cytokine-receptor
      recognition scheme.
PG  - 713-7
AB  - Granulocyte colony-stimulating factor (GCSF) is the principal growth factor
      regulating the maturation, proliferation and differentiation of the precursor
      cells of neutrophilic granulocytes and is used to treat neutropenia. GCSF is a
      member of the long-chain subtype of the class 1 cytokine superfamily, which
      includes growth hormone, erythropoietin, interleukin 6 and oncostatin M. Here we 
      have determined the crystal structure of GCSF complexed to the BN-BC domains, the
      principal ligand-binding region of the GCSF receptor (GCSFR). The two receptor
      domains form a complex in a 2:2 ratio with the ligand, with a
      non-crystallographic pseudo-twofold axis through primarily the interdomain region
      and secondarily the BC domain. This structural view of a gp130-type
      receptor-ligand complex presents a new molecular basis for cytokine-receptor
      recognition.
FAU - Aritomi, M
AU  - Aritomi M
AD  - Biomolecular Engineering Research Institute, Suita-city, Osaka, Japan.
FAU - Kunishima, N
AU  - Kunishima N
FAU - Okamoto, T
AU  - Okamoto T
FAU - Kuroki, R
AU  - Kuroki R
FAU - Ota, Y
AU  - Ota Y
FAU - Morikawa, K
AU  - Morikawa K
LA  - eng
SI  - PDB/1CD9
SI  - PDB/1PGR
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (Receptors, Granulocyte Colony-Stimulating Factor)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Crystallography, X-Ray
MH  - Granulocyte Colony-Stimulating Factor/*chemistry/metabolism
MH  - Humans
MH  - Mice
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Protein Conformation
MH  - Receptors, Granulocyte Colony-Stimulating Factor/*chemistry/metabolism
MH  - Sequence Homology, Amino Acid
EDAT- 1999/10/28 09:00
MHDA- 2001/03/23 10:01
CRDT- 1999/10/28 09:00
PHST- 1999/10/28 09:00 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/10/28 09:00 [entrez]
AID - 10.1038/44394 [doi]
PST - ppublish
SO  - Nature. 1999 Oct 14;401(6754):713-7. doi: 10.1038/44394.