PMID- 10537060
OWN - NLM
STAT- MEDLINE
DCOM- 19991105
LR  - 20091119
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 73
IP  - 5
DP  - 1999 Nov
TI  - Interleukin-6 activates signal transducer and activator of transcription and
      mitogen-activated protein kinase signal transduction pathways and induces de novo
      protein synthesis in human neuronal cells.
PG  - 2009-17
AB  - Interleukin-6 (IL-6) is involved in the pathophysiology of various diseases of
      the CNS. Because the molecular mechanism of action of this cytokine in human
      neurons is not well understood, we were interested in characterizing and defining
      a model system for IL-6-induced activation of signal transduction cascades,
      transcriptional activation, and protein synthesis in human neuronal cells. We
      show that IL-6 leads to transcriptional activation of signal transducer and
      activator of transcription 3 (STAT3) in human SH-SY5Y neuroblastoma cells.
      IL-6-induced activation and translocation of STAT3 and to a lesser degree STAT1
      but not STAT5 are demonstrated. STAT3 is phosphorylated on Tyr705 and Ser727
      residues on stimulation with IL-6, suggesting maximal activation of
      transcription. We also show IL-6-induced phosphorylation of p42/44
      mitogen-activated protein (MAP) kinase, providing evidence for MAP kinase pathway
      activation. The physiological relevance of our results is confirmed by
      IL-6-induced phosphorylation of key signaling proteins of both STAT and MAP
      kinase pathway in rat primary hippocampal neurons. Furthermore, de novo protein
      synthesis on IL-6 activation is demonstrated.
FAU - Schumann, G
AU  - Schumann G
AD  - Department of Psychiatry and Psychotherapy, University of Freiburg Medical
      School, Germany.
FAU - Huell, M
AU  - Huell M
FAU - Machein, U
AU  - Machein U
FAU - Hocke, G
AU  - Hocke G
FAU - Fiebich, B L
AU  - Fiebich BL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Interleukin-6)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (STAT1 Transcription Factor)
RN  - 0 (STAT1 protein, human)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (STAT3 protein, human)
RN  - 0 (Stat1 protein, rat)
RN  - 0 (Stat3 protein, rat)
RN  - 0 (Trans-Activators)
RN  - 17885-08-4 (Phosphoserine)
RN  - 21820-51-9 (Phosphotyrosine)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - DNA-Binding Proteins/*metabolism
MH  - Dimerization
MH  - Hippocampus/metabolism
MH  - Humans
MH  - Interleukin-6/*pharmacology
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Nerve Tissue Proteins/*biosynthesis
MH  - Neuroblastoma
MH  - Neurons/*metabolism
MH  - Phosphorylation
MH  - Phosphoserine/metabolism
MH  - Phosphotyrosine/metabolism
MH  - Rats
MH  - STAT1 Transcription Factor
MH  - STAT3 Transcription Factor
MH  - *Signal Transduction
MH  - Trans-Activators/*metabolism
MH  - Tumor Cells, Cultured
EDAT- 1999/10/28 00:00
MHDA- 1999/10/28 00:01
CRDT- 1999/10/28 00:00
PHST- 1999/10/28 00:00 [pubmed]
PHST- 1999/10/28 00:01 [medline]
PHST- 1999/10/28 00:00 [entrez]
PST - ppublish
SO  - J Neurochem. 1999 Nov;73(5):2009-17.