PMID- 10537036
OWN - NLM
STAT- MEDLINE
DCOM- 19991105
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 73
IP  - 5
DP  - 1999 Nov
TI  - The 5' untranslated regions of the rat A2A adenosine receptor gene function as
      negative translational regulators.
PG  - 1790-8
AB  - The rat A2A adenosine receptor (A2A-R) gene contains two promoters, P1 and P2,
      which produce transcript 1 and transcript 2, respectively. These transcripts
      differ in the lengths of their 5' untranslated regions (5'UTR1: 514 bp, initiated
      from P1; 5'UTR2: 221 bp, initiated from P2) but encode the same protein. In the
      present study, we demonstrate that transcript 2 is present in various tissues at 
      different levels, whereas transcript 1 is found only in the striatum. In the
      striatum, the level of transcript 2 is approximately 300-fold higher than that of
      transcript 1. The 5'UTR of both transcripts suppresses the expression of A2A-R
      and a firefly luciferase reporter gene at the translational level; this
      suppression is not observed after mutational inactivation of an "out-of-frame"
      upstream AUG codon. Translational suppression by the 5'UTR was also confirmed in 
      cells using a bicistronic strategy. Collectively, these data suggest that P2 is
      the major promoter of the rat A2A-R gene. The 5'UTR of the rat A2A-R gene exerts 
      an inhibitory effect on translation by an upstream open reading frame. Because
      the 5'UTR of the A2A-R gene possesses strong interspecies homology, translational
      suppression may be a general mechanism by which the expression of the A2A-R gene 
      is regulated.
FAU - Lee, Y C
AU  - Lee YC
AD  - Graduate Institute of Life Sciences, National Defense Medical Center, Taipei,
      Taiwan, ROC.
FAU - Chang, C W
AU  - Chang CW
FAU - Su, C W
AU  - Su CW
FAU - Lin, T N
AU  - Lin TN
FAU - Sun, S H
AU  - Sun SH
FAU - Lai, H L
AU  - Lai HL
FAU - Chern, Y
AU  - Chern Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Receptors, Purinergic P1)
RN  - 0 (Untranslated Regions)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - CHO Cells
MH  - Cell Line
MH  - Cricetinae
MH  - *Gene Expression Regulation
MH  - Genes
MH  - Glioma
MH  - Humans
MH  - Luciferases/genetics
MH  - Molecular Sequence Data
MH  - PC12 Cells
MH  - Polymerase Chain Reaction
MH  - *Protein Biosynthesis
MH  - Rats
MH  - Receptors, Purinergic P1/*genetics
MH  - Sequence Alignment
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - *Untranslated Regions
EDAT- 1999/10/28 00:00
MHDA- 1999/10/28 00:01
CRDT- 1999/10/28 00:00
PHST- 1999/10/28 00:00 [pubmed]
PHST- 1999/10/28 00:01 [medline]
PHST- 1999/10/28 00:00 [entrez]
AID - 10.1046/j.1471-4159.1999.0731790.x [doi]
PST - ppublish
SO  - J Neurochem. 1999 Nov;73(5):1790-8. doi: 10.1046/j.1471-4159.1999.0731790.x.