PMID- 10536173
OWN - NLM
STAT- MEDLINE
DCOM- 19991223
LR  - 20190516
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 15
IP  - 5
DP  - 1999 Nov
TI  - EXTL3/EXTR1 alterations in colorectal cancer cell lines.
PG  - 915-9
AB  - We previously demonstrated that metastasis-related tumor suppressor gene(s) may
      exist on chromosome 8p21-22 on allelotype analysis of early colorectal carcinomas
      (CRC) with lymph node metastasis. Here, we searched for target gene(s) in this
      chromosomal region in the UniGene database. The EXTL3 (also called EXTR1) gene
      was selected as a candidate because of its homology to EXT1 and EXT2, putative
      tumor suppressor genes. We screened 12 CRC cell lines for mutations by means of
      polymerase chain reaction (PCR)-single strand conformation polymorphism. Three
      cell lines showed EXTL3 mutations, all of which were located within exon 3 and
      caused amino acid substitutions. Reverse transcription-PCR analysis showed that
      the EXTL3 expression was lacking in 1 of the 12 colorectal cancer cell lines.
      Although there is still no definitive evidence that EXTL3 is a tumor suppressor
      gene for CRC, these data suggest that inactivation of the EXTL3 gene may at least
      offer a selective growth advantage for some CRC cell lines.
FAU - Arai, T
AU  - Arai T
AD  - First Department of Surgery, Medical Research Institute, Tokyo Medical and Dental
      University, Bunkyo-ku, Tokyo 113-8519, Japan.
FAU - Akiyama, Y
AU  - Akiyama Y
FAU - Nagasaki, H
AU  - Nagasaki H
FAU - Murase, N
AU  - Murase N
FAU - Okabe, S
AU  - Okabe S
FAU - Ikeuchi, T
AU  - Ikeuchi T
FAU - Saito, K
AU  - Saito K
FAU - Iwai, T
AU  - Iwai T
FAU - Yuasa, Y
AU  - Yuasa Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (EXTL3 protein, human)
RN  - 0 (Proteins)
RN  - EC 2.4.1.- (N-Acetylglucosaminyltransferases)
SB  - IM
MH  - Adenomatous Polyposis Coli/genetics
MH  - Amino Acid Substitution
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 8
MH  - Colonic Neoplasms/*genetics
MH  - Colorectal Neoplasms/*genetics
MH  - Exons
MH  - Exostoses, Multiple Hereditary/genetics
MH  - Humans
MH  - *N-Acetylglucosaminyltransferases
MH  - *Point Mutation
MH  - Polymorphism, Single-Stranded Conformational
MH  - Proteins/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Cells, Cultured
EDAT- 1999/10/28 00:00
MHDA- 1999/10/28 00:01
CRDT- 1999/10/28 00:00
PHST- 1999/10/28 00:00 [pubmed]
PHST- 1999/10/28 00:01 [medline]
PHST- 1999/10/28 00:00 [entrez]
AID - 10.3892/ijo.15.5.915 [doi]
PST - ppublish
SO  - Int J Oncol. 1999 Nov;15(5):915-9. doi: 10.3892/ijo.15.5.915.