PMID- 10536163
OWN - NLM
STAT- MEDLINE
DCOM- 19991228
LR  - 20190501
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 27
IP  - 22
DP  - 1999 Nov 15
TI  - CUG repeat binding protein (CUGBP1) interacts with the 5' region of C/EBPbeta
      mRNA and regulates translation of C/EBPbeta isoforms.
PG  - 4517-25
AB  - The transcription factor CCAAT/enhancer binding protein beta, C/EBPbeta, plays a 
      significant role in the regulation of hepatocyte growth and differentiation. A
      single mRNA coding for C/EBPbeta produces several protein isoforms. Two pathways 
      for generation of low molecular weight C/EBPbeta isoforms have been described:
      specific proteolytic cleavage and initiation of translation from different AUG
      codons of C/EBPbeta mRNA. A truncated C/EBPbeta isoform, LIP, is induced in rat
      livers in response to partial hepatectomy (PH) via the alternative translation
      mechanism. Here we present evidence that CUG repeat binding protein, CUGBP1,
      interacts with the 5' region of C/EBPbeta mRNA and regulates translation of
      C/EBPbeta isoforms. Two binding sites for CUGBP1 are located side by side between
      the first and second AUG codons of C/EBPbeta mRNA. One binding site is observed
      in an out of frame short open reading frame (sORF) that has been previously shown
      to regulate initiation of translation from different AUG codons of C/EBPbeta
      mRNA. Analysis of cytoplasmic and polysomal proteins from rat liver after PH
      showed that CUGBP1 is associated with polysomes that translate low molecular
      weight isoforms of C/EBPbeta. The binding activity of CUGBP1 to the 5' region of 
      C/EBPbeta mRNA shows increased association with these polysomal fractions after
      PH. Addition of CUGBP1 into a cell-free translation system leads to increased
      translation of low molecular weight isoforms of C/EBPbeta. Our data demonstrate
      that CUGBP1 protein is an important component for the regulation of initiation
      from different AUG codons of C/EBPbeta mRNA.
FAU - Timchenko, N A
AU  - Timchenko NA
AD  - Huffington Center on Aging, Department of Pathology, Baylor College of Medicine, 
      Houston, TX 77030, USA. nikolait@bcm.tmc.edu
FAU - Welm, A L
AU  - Welm AL
FAU - Lu, X
AU  - Lu X
FAU - Timchenko, L T
AU  - Timchenko LT
LA  - eng
GR  - AG00756/AG/NIA NIH HHS/United States
GR  - AR10D44387-01/AR/NIAMS NIH HHS/United States
GR  - HL543-13/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (5' Untranslated Regions)
RN  - 0 (CCAAT-Enhancer-Binding Protein-beta)
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (CELF1 Protein)
RN  - 0 (CELF1 protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Ribonucleoproteins)
SB  - IM
MH  - 5' Untranslated Regions/*metabolism
MH  - Animals
MH  - Binding Sites
MH  - CCAAT-Enhancer-Binding Protein-beta
MH  - CCAAT-Enhancer-Binding Proteins
MH  - CELF1 Protein
MH  - Cell-Free System
MH  - DNA-Binding Proteins/*genetics
MH  - Gene Expression Regulation
MH  - HeLa Cells
MH  - Humans
MH  - Liver Regeneration
MH  - Nuclear Proteins/*genetics
MH  - Open Reading Frames
MH  - Polyribosomes/metabolism
MH  - Protein Biosynthesis
MH  - Protein Isoforms/genetics
MH  - RNA, Messenger/*metabolism
MH  - RNA-Binding Proteins/*metabolism
MH  - Rabbits
MH  - Rats
MH  - Repressor Proteins/genetics
MH  - Ribonucleoproteins/*metabolism
MH  - Trinucleotide Repeats/physiology
PMC - PMC148737
EDAT- 1999/10/28 00:00
MHDA- 1999/10/28 00:01
CRDT- 1999/10/28 00:00
PHST- 1999/10/28 00:00 [pubmed]
PHST- 1999/10/28 00:01 [medline]
PHST- 1999/10/28 00:00 [entrez]
AID - gkc660 [pii]
AID - 10.1093/nar/27.22.4517 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1999 Nov 15;27(22):4517-25. doi: 10.1093/nar/27.22.4517.